6-Title: Molecular characerization, polymorphism and association study of lactoferrin gene     with milk production and somatic cell traits in crossbred (Bos indicus x Bos taurus) cattle

Authors: Ratan K Choudhary, Pushpendra Kumar, Arjava Sharma and Tarun K Bhattacharya

Source: Ruminant Science (2014)-3(2):157-162

How to cite this manuscript: Choudhary RK, Kumar Pushpendra, Sharma Arjava and Bhattacharya TK (2014). Molecular characerization, polymorphism and association study of lactoferrin gene with milk production and somatic cell traits in crossbred (Bos indicus x Bos taurus) cattle. Ruminant Science 3(2):157-162.

Abstract

Lactoferrin is an anti-microbial protein found in exocrine secretions like milk, saliva, tear of animals and human, and confers immunity. A study was conducted to elucidate gene sequence of lactoferrin in Indian Sahiwal X Holstein Friesian (crossbred) cattle and to explore polymorphism and its possible association with phenotypic traits like milk production and somatic cell counts. The total length of lactoferrin cDNA was found to be 2127 bp. The similarity with taurus cattle, goat, human and pig human were 99.5%, 94.7%, 57.8%, and 31.2%, respectively. Polymorphism study of exon 4 was carried out using single stranded confirmation polymorphism (SSCP) and showed four alleles namely A, B, C and D with respective frequencies of 0.40, 0.33. 0.17 and 0.10 in crossbred cattle population. Three genotypes were found in this population where the most predominant (0.67) was AB genotype. Other two genotypes AC and CD were estimated as 0.20 and 0.13, respectively. The genotypes showed significant effect (P<0.05) on total milk yield, lactation length and somatic cell count. This study suggests, crossbred cattle lactoferrin gene has significant variation with other species and polymorphism of exon 4 region of gene is significantly associated with certain milk production traits.

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