13-Title: Genetic polymorphism of alpha-lactalbumin and beta-lactoglobulin genes in Marathwadi buffalo
Authors: Akshay Joshi, PS Bankar, SV Kuralkar, S Sajid Ali, SG Deshmukh and Prajakta Kuralkar
Source: Ruminant Science (2019)-8(2):205-208
How to cite this manuscript: Joshi Akshay, Bankar PS, Kuralkar SV, Ali S Sajid, Deshmukh SG and Kuralkar Prajakta (2019). Genetic polymorphism of alpha-lactalbumin and beta-lactoglobulin genes in Marathwadi buffalo. Ruminant Science 8(2):205-208.
The present research work was undertaken to detect the genetic polymorphism of major whey protein genes, i.e. Alpha-Lactalbumin (alpha-LA) and Beta-Lactoglobulin (ß-LG) genes in Marathwadi buffaloes using PCR-RFLP technique and to observe the association of polymorphism with milk components viz. protein, fat and lactose contents in Marathwadi (N=30) buffaloes. The available data on total lactation milk yield was collected from 30 unrelated Marathwadi buffaloes maintained at Marathwadi Buffalo Farm, College of Veterinary and Animal Sciences, Udgir. The mean values for protein, fat and lactose content in Marathwadi buffalo ranged from 3.8-4.3%, 3.9-5.8% and 3.7-4.2%, respectively. The total yields for protein, fat and lactose were estimated by Test Interval Method and ranged from 19.66 kg to 30.52 kg, 21.99 kg to 31.11 kg and 18.68 kg to 28.65 kg, respectively. The PCR-RFLP was carried out using reported primers of alpha-LA (309 bp) and ß-LG gene (247 bp), followed by restriction enzyme digestion MspI and HaeIII, respectively. The digested PCR products exhibited a single restriction pattern for both the genes. Two fragments of 220 bp and 89 bp were yielded for alpha-LA gene, leading to a monomorphic pattern of BB genotype. Similarly, three fragments of 148, 99 and 74 bp were yielded for ß-LG gene, showing the monomorphic pattern of AB genotype, respectively. All the samples screened revealed absence of polymorphism in alpha-LA and ß-LG genes; therefore, the association study between these genes and milk components was not performed. The study highlighted the need for screening a larger population of Marathwadi buffaloes for detecting the genetic polymorphism and its association with economically essential milk traits, which will provide a robust platform for marker-assisted selection.
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