In vitro assessment of acaricidal activity of garlic cloves and papaya leaves against deltamethrin and cypermethrin

20-Title: In vitro assessment of acaricidal activity of garlic cloves and papaya leaves against deltamethrin and cypermethrin susceptible Rhipicephalus (Boophilus) microplus

Authors: KP Shyma, Veer Singh, Jay Prakash Gupta and Mahesh M Pawar

Source: Ruminant Science (2019)-8(2):233-236.

How to cite this manuscript: Shyma KP, Singh Veer, Gupta Jay Prakash and Pawar MM (2019). In vitro assessment of acaricidal activity of garlic cloves and papaya leaves against deltamethrin and cypermethrin susceptible Rhipicephalus (Boophilus) microplus. Ruminant Science 8(2):233-236.



In the present study, the tick samples were collected from organized and unorganized farms located at Dhanera Taluka, Banaskantha districts of Gujarat state for assessment of acaricidal activity. In vitro tests, Larval Packet Test (LPT) and Larval Immersion Test (LIT) were used to see the effectiveness of both chemicals (deltamethrin and cypermethrin) and herbal acaricides (garlic cloves and papaya leaves). LC50 (1.11-LPT; 0.203-LIT), LC95 (56.03-LPT; 26.5-LIT), resistance factor of deltamethrin and cypermethrin against R. (B.) microplus were established.  LC50 (25.7-LPT; 0.72-LIT) of herbal acaricides were also ascertained. The results indicated that Dhanera isolates of R. (B.) microplus were susceptible to both the synthetic acaricides. It was further noticed that LIT is more sensitive for the detection of status of acaricidal resistance. Referring to the plant extracts, both garlic cloves and papaya leaves were able to cause significant mortality in the larvae. However, garlic cloves were more efficient when compared to papaya leaves. Both the botanicals can be used as potential acaricides and further investigation are suggested to identify the active principles in these extracts.


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