10-Title: Detection of exou and exos genes among Pseudomonas aeruginosa isolates of animal and human origin

Authors: Rahul Agarwal, Sunil Maherchandani and Dharam Gopal Gupta

Source: Ruminant Science (2013)-2(1):45-49.

How to cite this manuscript: Agarwal Rahul, Maherchandani Sunil and Gupta Dharam Gopal (2013). Detection of exou and exos genes among Pseudomonas aeruginosa isolates of animal and human origin. Ruminant Science 2(1):45-49.

Abstract

A total of 35 isolates of P. aeruginosa were obtained from 119  clinical samples of animal and human origin were subjected to genotypic charecterization using exoS and exoU gene responsible for ADP-ribosilating enzyme activity and phospholipase activity  respectively  acting as major genes responsible for virulent activity. Both these genes mostly are mutually exclusive type III effector genes. Out of 35 clinical isolates, 22(62.85%) were found to be positive for exoS gene and 18(51.42%) were found to be positive for exoU gene. Of these 22 isolates having exoS, there were 11 (31.42%) isolates which had both exoS and exoU genes which showed that exoS and exoU genes do not seem to be essentially mutually exclusive. High percentage of isolates were found to have either both the genes or none of them.

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