11-Title: Cloning of the putative heparin binding site of goat vitronectin
Authors: S Murugavel, HM Manu, I Karuna devi, TL Prasanth, Manish Mahawar and P Joshi
Source: Ruminant Science (2013)-2(1):51-53.
How to cite this manuscript: Murugavel S, Manu HM, Devi I Karuna, Prasanth TL, Mahawar Manish and Joshi P (2013). Cloning of the putative heparin binding site of goat vitronectin. Ruminant Science 2(1):51-53.
Vitronectin (VN) is a matrix glycoprotein which plays a significant role in bacterial pathogenesis through its various domains. The heparin binding region of human VN has been studied extensively, whereas details about this region in animal VN is lacking. This domain is known to have roles in various physiological processes. The current study describes cloning and sequencing of the heparin binding region of goat VN. This region was PCR amplified from goat cDNA which yielded an expected product size of 120 bp approximately. Subsequently, it was cloned in an expression vector and sequenced. The sequence similarity was analysed by nucleotide BLAST. This construct can be used subsequently for expression and other functional studies.
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