15-Title: Cryopreservation of Bhadawari buffalo bull spermatozoa in tris and citrate based extenders
Authors: Alok K Sahrawat, Atul Saxena and Sanjay K Mishra
Source: Ruminant Science (2013)-2(1):67-70.
How to cite this manuscript: Sahrawat Alok K, Saxena Atul and Mishra Sanjay K (2013). Cryopreservation of Bhadawari buffalo bull spermatozoa in tris and citrate based extenders. Ruminant Science 2(1):67-70.
The aim of the present experiment was to study the effect of glycerolated egg yolk Tris (GEYT) and glycerolated egg yolk citrate (GEYC) extenders on freezability of Bhadawari bull semen. Semen samples (n=56) from four Bhadawari bulls aged between 4.5-5 years were collected using artificial vagina. The mean ejaculate volume, mass motility, concentration, total sperm concentration, progressive motility, livability and total sperm abnormalities were recorded as 2.76±0.11 ml, 3.41±0.06, 2271.20±69.92 millions/ml, 6374.16±353.11 millions, 75.39±.48%, 85.76±1.11% and 3.82±.24% , respectively. After thawing, the progressive motility was recorded as 56.49%, live sperm as 72.27 %, HOS as 69.16 %, per cent intact acrosome as 71.31% and total sperm abnormalities as 3.52% in Tris extended semen. The respective values in Citrate based extended semen were 54.56, 69.28, 67.36, 67.58 and 4.27 per cent. These findings indicate the superiority of GEYT over GEYC extender as it preserves the maximum seminal attributes.
Anel L, De Paz P, Alvarez M, Chamorro CA, Boixo JC and Manso A (2003). Field and in vitro assay of three methods for freezing ram semen. Theriogenology 60:1293-1308.
Byrne GP, Lonergan P, Wade M, Duffy P, Donavan A and Hanrahan JP (2000). Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro. Animal Reproduction Science 62:265-275.
Chinnaiya GP and Ganguli NC (1980). Freezability of buffalo semen in different extenders. Zb1 Veterinary Medicine A 27:563-568.
Fabbrocini A, Del Sorbo C, Fasano G, Martusciello MR and Sansone G (1995). Effect of cryoprotectant addition mode on freezed-thawed Bubalus bubalis semen. Proceeding of 30th Symposium on Reproduction and Animal Breeding: Advance and Strategy, Milano, Italy vol. 1 pp 373-374.
Foote RH (1970). Fertility of bull semen at high extension rates in Tris buffer extenders. Journal of Dairy Science 53:1475-7.
Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM (1966). Hydrogen ion buffers for biological research. Biochemistry 5:467-477.
Hafez ESE (1987). Semen evaluation. In: Reproduction in Farm Animal. Lea and Febiger, Philadelphia, pp 455-480.
Kumar AM (1993). Sexual behaviour pattern in Sahiwal and Murrah bulls. MSc Thesis submitted to NDRI Deemed University, Karnal, India.
Lodhi LA, Zubair M, Quresshi ZI, Ahmad I and Jamil H (2008). Correlation between Hypo-osmotic swelling test and various conventional semen evaluation parameters in fresh Nili-Ravi buffalo and Sahiwal cow bull semen. Pakistan Veterinary Journal 28(4):186-188.
Matharoo JS and Singh M (1980). Revivability of buffalo spermatozoa after deep freezing the semen using various extenders. Animal Breeding Abstract 52:392.
Mazur P (1984). Freezing of living cells: Mechanisms and implications. American Journal of Physiology 247:C125-C142.
Medeiros CM, Forell F, Oliveira AT and Rodrigues JL (2002). Current status of sperm cryopreservation: Why isn’t better. Theriogenology 57:327-344.
Prasad JK, Kumar S, Mohan G, Shankar U and Agarwal SK (1999). Hypo-osmotic swelling test (HOST) and its response in fresh and freeze-thawed semen. Indian Journal of Animal Science 69:766-769.
Shabd R (1998). MVSc Thesis submitted to GBPUAT, Pantnagar,UP.
Salamon S and Maxwell W (2000). Storage of ram semen. Animal Reproduction Science 62:77-111.
Salisbury GW (1941). Preservation of bovine spermatozoa in yolk citrate diluents and field result from its use. Journal of Dairy Science 24:905-910.
Samad SA (1985). Diluters of buffalo semen. Processing and use of buffalo semen. Animal Science Institute NARC, Islamabad, 34-46.
Sekoni V and Oyedipe EO (1995). Embryo transfer technology and its possible application to the Nigerian livestock industry. In: Proceedings of the Symposium on Chemistry and Biotechnology for National Development 16-19.
Snedecor GW and Cochran WG (1973). Statistical Methods. 6th Edn, Oxford and IBH Publishing Co, Bombay.
Tomar NS (1984). Artificial Insemination and Reproduction of Cattle and Buffaloes. 3rd Edn Saroj Prakashan, Allahabad.
Tuli RK, Singh M and Matharoo JS (1981). Effect of different equilibration times and extenders on deep freezing of buffalo semen. Theriogenology 16:99-104.
Watson PF (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assesment of their post-thawing function. Reproduction Fertility Development 7:871-891.