Title: In-vitro maturation of buffalo oocytes IN TCM-199 media supplemented with follicular fluid, hormones and antioxidants

Authors: AK Sharma, SS Chaudhary and Gopal Puri

Source: Ruminant Science (2017)-6(1):13-18.

Cite this reference as: Sharma AK, Chaudhary SS and Puri Gopal (2017). In-vitro maturation of buffalo oocytes in TCM-199 media supplemented with follicular fluid, hormones and antioxidants. Ruminant Science 6(1):13-18.

Abstract

The aim of the present study was to evaluate the effects of follicular fluid, hormones and antioxidants (cysteamine and α-mercaptoethanol) in medium 199 for in vitro maturation of Surti and Mehsani breed buffalo oocytes. The culturable oocytes (1174) retrieved from buffalo ovaries by aspiration technique were used for in vitro maturation. Oocyte maturation rate was evaluated on the basis of visual assessment and degree of cumulus expansion after 24 hrs of in vitro culture at 38.5 ºC and 5% CO2 with 95 % relative humidity in CO2 incubator and were graded degree 0, 1 and 2 on the basis of cumulus expansion. Results of the study showed that the overall mean recovery rate of oocytes/ ovary was 2.20±0.08 while the culturable grade (Grade A and B combined) was 1.35±0.06, mean retrieval percentage of culturable COCs was 60.83±0.84. The percentage of matured oocytes for degree 2, 1 and 0 were 58.15±1.24, 21.99±0.70 and 19.86±1.19, respectively. The overall mean maturation percentage (degree 1 and 2) was 80.14±1.20. The present finding of experiment led to the conclusion that medium 199 supplemented with follicular fluid, hormones and antioxidants like cysteamine and α-mercaptoethanol @ 200mM each, improved the in vitro maturation rate of buffalo oocytes.

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