Histo-chemical study of uterus during different stages of pregnancy in goat (Capra hircus)

Title: Histo-chemical study of uterus during different stages of pregnancy in goat (Capra hircus)

Authors: Vijay Kumar, SP Singh, MM Farooqui, Chetna Gangwar, Prabhakar Kumar and Ajay Prakash

Source: Ruminant Science (2018)-7(1):67-70.

Cite this reference as: Kumar Vijay, Singh SP, Farooqui MM, Gangwar Chetna, Kumar Prabhakar and Prakash Ajay (2018). Histo-chemical study of uterus during different stages of pregnancy in goat (Capra hircus). Ruminant Science 7(1):67-70.

Abstract

The present study was conducted on 18 gravid uteri obtained from healthy goats of non-descript breed procured from local slaughter house. The uteri were divided into three groups according to their gestational ages as Gr I (0-50 days), Gr II (51-100 days) and Gr  III (101 till term). The Perodic acid Schiff’s (PAS), acid mucopolysaccharides (AMPs), lipase and Feulgen reaction were studied during different stages of pregnancy.  The different part of the endometrium, endometrial glands, blood vessels of the stratum vasculare and perimetrium showed PAS positive activity which increased with the advancement of the pregnancy. The activity of AMPs was mild   to moderate and was more pronounced during initial stage of pregnancy. Mild to intense lipase activity was observed in the different parts of the endometrium, endometrial glands and mild activity in the blood vessels of the stratum vasculare and perimetrium during all stages of pregnancy. Mild to moderate Feulgen reaction was observed in the endometrium and moderate to intense in the endometrial glands. Myometrium showed very mild PAS positive, AMPs, lipase and Feulgen reaction during all the three stages of pregnancy.

 

References

Al-Ramadan SY (2014). Camel endometrium: Light-microscopic and ultrastructural features related to pregnancy. Ruminant Science 3(2):129-140.

Bachhawat BK, Balasubrarnanian KA, Balasubrarnanian AS, Singh M, George E and Chandrasekaran EV (1972). Chemistry and metabolism of glycosaminoglycons of the nervous system. Advances in Experimental Medicine and Biology 25:51-71.

Chandra G (1973). Gross histological and histochemical observation on the female reproductive organs of buffalo (Bubalus bubalis). PhD Thesis submitted to Agra University, Agra.

Christopher J and Adam HB (2010). The adverse effects of obesity on conception and implantation. Reproduction 140:347-364.

Devenport HA (1960). Histological and histochemical techniques. WB Saunders Company, Philadelphia.

Duncan DA, Mazur MT and Younger JB (1998). Glycoconjugate distribution in normal human endometrium: Lectin histochemical study. International Journal of Gynecological Pathology 7:236-241.

Eurell JA and Frappier BL (2006). Dellmann’s Textbook of Veterinary Histology. 5th Edn, UK, Blackwell. pp 134-152.

Goding JR (1972). The demonstration that PGF2alpha is the uterine luteolysin in the ewe. Journal of Reproduction and Fertility 38:261-272.

Harvey EB (1959). Ageing and foetal development. In: Reproduction in Domestic Animals. Eds: H Cole and PT Eupps. Academic Press Inc, New York.

Lindhard A,  Bentin-Lay U, Ravn V, Islin  H, Hviid T,  Rex S,  Bangsboll S and Sorenson S (2002). Biochemical evaluation of endometrial functional the time of implantation. Fertility and Sterility 78:221-233.

Luna LG (1968). Manual of Histological Staining Methods of the Armed Forces Institute of Pathology. 3rd Edn, McGraw Hill Book Company, New York, USA.

Murphy CR and Turner VF (1991). Glycocalyx carbohydrates of uterine epithelial cells increase during early pregnancy in the rats. Journal of Anatomy 177:109-115.

Qureshi AS, Mohsin M and Rehan S (2015). Effect of parity on gross and microscopic structure of uterus in teddy goats (Capra hircus). Ruminant Science 4(2):133-136.

Shahrooz R, Razi M and Babai A (2013). Histochemical study of river buffalo’s uterine endometrium in follicular and luteal phases Iranian Journal of Veterinary Research 14(4):320-326.

Shemesh M,  Bensadoun A and Hansel W (1976). Lipoprotein lipase activity in the bovine corpus luteum during the estrous cycle and early pregnancy. Proceedings of the Society for Experimental Biology and Medicine 151:667-669.

Singh Y, Sharma DN and Dhingra LD (1979). Morphogenesis of the testis in goat. Indian Journal of Animal Science 49:925-931.