Title: Prevention and evaluation of post-caesarean uterine  adhesions in torsion affected Mehsana buffaloes

Authors: PM Chauhan, VK Sharma, HC Nakhashi and TV Sutaria

Source: Ruminant Science (2019)-8(1):35-38.

Cite this reference as: Chauhan PM, Sharma VK, Nakhashi HC and Sutaria TV (2019). Prevention and evaluation of post-caesarean uterine adhesions in torsion affected Mehsana buffaloes. Ruminant Science 8(1):35-38.

 Abstract

Twenty seven torsion affected Mehsana buffaloes equally divided in three groups were studied for prevention of uterine adhesions by applying 1% Sodium Carboxy Methyl Cellulose (SCMC) @ 7 ml/kg b. wt. (Group-A), 2% SCMC @ 14 ml/kg b. wt (Group-B) and the hyaluronic acid @ 0.2 mg/kg b. wt. (Group-C) on the sutured uterus following caesarean whereas six buffaloes did not receive any treatment and were kept as control (Group-D). The SCMC was also advocated intraperitoneal to achieve hydro-floatation effect. The efficacy of treatments was assessed clinically by presence and severity of adhesions on day 45-50 post-caesarean as well as by estimation of blood plasma fibrinogen initially on day of hospitalization, immediately after delivery of fetus followed by 24 h later to fetal delivery and thereafter on day 3, 5 and 10 of post-caesarean. 1% SCMC and hyaluronic acid treatments were equally effective in preventing post-operative uterine adhesions in 42.86 % buffaloes with an additional advantage of higher percentage of mild type adhesions (75%) resulted from hyaluronic acid group than those of 1% SCMC group (50%). The plasma fibrinogen also did not differ significantly between these two groups specially after 24 h of fetal delivery (265.14±89.87 and 566.43±214.41 mg/dl), day 3 (442.00±219.23 and 993.43±262.67 mg/dl and subsequently on day 5 (631.00±184.00 and 726.86±187.80 mg/dl) whereas in 2% SCMC group, the fibrinogen concentration remained significantly higher than other treatment and control groups  besides a many fold rise in this group on day 3 and 5 (2452.86±594.90 and 1729.00±343.55 mg/dl) as compared to its preceding concentration after 24 h of fetal delivery (892.71±185.15 mg/dl). It is concluded that hyaluronic acid and 1% SCMC treatment prevented the post-operative uterine adhesions equally and efficiently in torsion affected buffaloes.

References

Dhindsa SS, Dhaliwal GS and Ghuman SPS (2010). Prevention of uterine adhesion formation following caesarean section in bovine. Indian Journal of Animal Science 80(7):626-628.

Dhindsa SS, Dhaliwal GS and Ghuman SPS (2008). Biochemical alterations in bovines due to caesarean operation with reference to suture material and intra-peritoneal lubricant. Indian Journal of Animal Science 78:1082-1085.

Eckersall PD and Bell R (2010). Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine. Veterinary Journal 185:23-27.

Hendersons RA (1982). Conrolling peritoneal adhesions. Veterinary Surgery 11:30-33.

Holtz G and Baker ER (1980). Inhibition of peritoneal reformation after analysis with thirty-two per cent dextram 70. Fertility and Steritility 34:394-96.

Karthick C, Selvaraju M, Napolean RE, and Doraisamy KA (2016).  Haematological changes during uterine torsion and detorsion in buffaloes. Indian Veterinary Journal 93(10):22-25.

Leach RE, Burns JW, Dawe EJ, SmithBarbour MD and Diamond MP (1998). Reduction of postsurgical adhesion formation in the rabbit uterine horn model with use of hyaluronate/ carboxymethylcellulose gel. Fertility and Sterility 69(3):415-418.

McSherry BJ, Horney FD and Degroot JJ (1970). Plasma fibrinogen levels in normal and sick cows. Canadian Journal of Comparative Medicine 34:191.

Moll HD, Schumacher J, Wright JC and Spano JS (1991). Evaluation of sodium carboxy methyl cellulose for prevention of experimentally induced abdominal adhesions in ponies. American Journal of Veterinary Science 52:88-91.

Moll HD, Wolfe DF, Schumacher J and Wright JC (1992). Evaluation of sodium carboxy methyl cellulose for prevention of adhesions after uterine trauma in ewes. American Journal of Veterinary Research 58:1454-1456.

Murata H, Shimada N and Yoshioka M (2004). Current research on acute phase proteins in veterinary diagnosis: An overview. Veterinary Journal 168:28-40.

Patel SC, Patel MP, Desai SB and Nakhashi HC (2012). Haematic mummification associated with uterine torsion in a Kankrej cow. Ruminant Science 1(2):199-200.

Prathaban S and Gnanaprakasam V (1990). Study on plasma fibrinogen levels on Indian crossbred cows in health and disease. Indian Journal of Animal Science 58:293-97.

Purohit GN, Mehta JS, Sharma Sunanda, Shekher Chandra, Kumar Pramod , Kumar Amit, Raiya Sunil, Ruhil Swati and  Gaur Mitesh (2013). Uterine torsion in buffaloes: A retrospective analysis of 52 cases. Ruminant Science 2(2):219-222.

Roberts SJ (1971). Veterinary Obstetrics and Genital Diseases. 2nd Edn, CBS Publishers and Distributors, New Delhi.

Saraswat Chandrashekher, Dholpuria Sandeep, Jhamb Dinesh, Gaur Mitesh and Sharma  Sunandha (2015). Clinico-obstetrical approaches on uterine torsion cases in Surti buffalo of tribal area of southern Rajasthan. Ruminant Science 4(2):225-230.

Schalm CW, Jain NC and Carroll EJ (1975). Veterinary Haematology. 3rd Edn, Lea and Febiger, Philadelphia.

Singh J (1996). Studies on the uterine adhesions following caesarean operation in buffaloes. MVSc Thesis submitted to PAU, Ludhiana, India.

Singh J, Dhaliwal GS, Jagir S and Sharma RD (2002). Efficacy of different treatments in preventing uterine adhesions following caesarean section to relieve dystocia in buffaloes. Indian Journal of Animal Science 72:648-651.

Snedecor GW and Cochran WG (1994). Statistical Methods. 8th Edn, Iowa State University Press, Ames, Iowa.

Sutaria TV, Kapadiya PS, Nakhashi HC, Chauhan PM, Suthar BN and Sharma VK (2015). Study on treatment efficacy of uterine torsion in mehsana buffaloes. Ruminant Science 4(1):109-111.

Wuijckhuise-Sjouke LA (1984). Plasma fibrinogen concentration as a indicator of the presence and severity of inflammatory disease in horses and cattle. Tijdschrift voor Diegeeskunda 109:809-72.