A study on embryo production through in-vitro fertization

A study on embryo production through in-vitro fertization of caprine oocytes

Title: A study on embryo production through in-vitro fertization of caprine oocytes

Authors: JK Agrawal, SD Kharche, A Saxena, S Yadav and RP Panday

Source: Ruminant Science (2017)-6(2):333-336.

Cite this reference as: Agrawal JK, Kharche SD, Saxena A, Yadav S and Panday RP (2017). A study on embryo production through in-vitro fertization of caprine oocytes. Ruminant Science 6(2):333-336.

 

Abstract

The objective of the work was to study the cleavage rate of in-vitro fertilized embryos of caprine oocytes. A total of 3490 oocytes were retrieved from 812 caprine ovaries of abattoir origin. Recovery rate of oocytes per ovary was recorded 4.3. Oocytes were matured in-vitro in TCM-199 containing supplements for 27 hr in humidifier atmosphere of 5% CO2 at 38.5 ºC in CO2 incubator.  Maturation rate was 89.025 per cent. The results of the present study revealed that the overall cleavage rate through in-vitro fertilization was 14.8%. Out of total cleaved embryos, 134 two cell stage embryos were used for tetraploid embryo production. Developmental stages of remaining cleaved embryos were recorded (2 cell; 18.40%, 4 cell; 9.81%, 8-16 cell; 4.29%, morula; 0.92%, blastocysts).

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