Genotyping of Osmanabadi goat at FecX and FecB LOCI

Genotyping of Osmanabadi goat at FecX and FecB LOCI

Title: Genotyping of Osmanabadi goat at FecX and FecB LOCI

Authors: KG Bhave, MP Sawane, PT Wagh, KA Zunjarrao, VD Pawar and AY Doiphode

Source: Ruminant Science (2017)-6(2):207-214.

Cite this reference as: Bhave KG, Sawane MP, Wagh PT, Zunjarrao KA, Pawar VD and Doiphode AY (2017). Genotyping of Osmanabadi goat at FecX and FecB LOCI. Ruminant Science 6(2):207-214.


Prolificacy is one of the most important economical traits of goat. It is affected by various genetic and non-genetic parameters in small ruminants. We have carried out genotyping of Osmanabadi goats of either sex (n=160, 152 females and 08 males) at FecX loci (FecXI, FecXH, FecXB, FecXG and FecXL) of the BMP15 gene with T-ARMS-PCR. FecB/ Booroola locus of BMPR1B gene was, however genotyped using AvaII PCR-RFLP method. T-ARMS-PCR products of FecX loci (FecXI, FecXH and FecXL) and PCR-RFLP of FecB locus exhibited wild type alleles. FecXB and FecXG loci could amplify only control fragment, were subjected for direct sequencing of the control fragment. Sequencing revealed wild type alleles at nucleotide position 213 (G) of FecXB and at position 86 (C) FecXG locus. PCR-RFLP of FecB PCR products revealed a single band of 190 bp representing normal non-carrier animals. It indicated lack of FecX (BMP15) and FecB/ Booroola (BMPR1B) polymorphism in tested Osmanabadi goats. The prolificacy in Osmanabadi goats is therefore found to be independent of these established ovine fecundity gene polymorphisms.


Abulyazid I, Abdalla MS, Sharada HM and Hassanin WF (2011). Prolificacy detection in Egyptian sheep using RFLP-Specific PCR. Egyptian Academic Journal of Biological Sciences 3(1):1-4.

Abdel-Rahman SM, Mustafa YA, AbdErrasool HA, ElHanafy AA and Elmaghraby AM (2013). Polymorphism in BMP-15 gene and its association with litter size in Anglo-Nubian goat. Biotechnology in Animal Husbandry 29(4):675-683.

Ahlawat S, Sharma R and Maitra A (2013). Screening of indigenous goats for prolificacy associated DNA markers of sheep. Gene 517:128-131.

Al-Thabhawee AH, Samaka HM and Kadhim HM (2014). Detection of (FecB) gene polymorphism in local sheep breed at different area of Iraq. MRVSA 3(1):15-20.

Amiri S, Rahimi G and Vatankhah M (2008). No incidence of allelic mutation in Booroola (FecB) and Inverdale (FecXI) genes in Lori-Bakhtiari sheep breed. Proceedings of the 5th National Biotechnology Congress of Iran, Tehran, Iran. 495.

Asadpour, R, Jafari-Joozani R, Alijani S and Mahmodi H (2012). Detection of polymorphism in booroola gene (FecB) and its association with litter size in zel sheep breed in Iran. Slovak Journal of Animal Science 45(2): 63-66.

Banerjee GC (1998). A Textbook of Animal Husbandry. 8th Edn, Oxford and IBH Publishing: 942-943.

Cai-lan J, Ming-xing C, Jin-yu W, Li F and Su-cheng Y (2007). Cloning and sequence analysis of Exon 2 of BMP15 gene in Jining Grey Goats. Chinese Journal of Animal Husbandry and Veterinary Medicine 34:52-55.

Davis GH, Balakrishnan L, Ross IK, Wilson T, Galloway SM, Lumsden BM, Hanrahan JP, Mullen M, Mao XZ and Wang GL (2006). Investigation of the Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Animal Reproduction Science 92:87-96.

Davis GH, Galloway SM, Ross IK, Gregan SM, Ward J, Nimbkar BV, Ghalsasi PM, Nimbkar C, Gray GD, Subandriyo, Inounu I, Tiesnamurti B, Martyniuk E, Eythorsdottir E, Mulsant P, Lecerf F, Hanrahan JP, Bradford GE and Wilson T (2002). DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biology of Reproduction 66(6):1869-1874.

Davis GH (2005). Major genes affecting ovulation rate in sheep. Genetics Selection Evolution l37: S11-S23.

Davis GH, Montgomery GW, Allison AJ, Kelly RW and Bray AR (1982). Segregation of a major gene inluuencing fecundity in progeny of Booroola sheep. New Zealand Journal of Agricultural Research 25:525-529.

El-Hanafy AA and El-Saadani MA (2009). Fingerprinting of FecB gene in five Egyptian sheep breeds. Biotechnology and Animal Husbandry 25:205-212.

Falconer DS and Mackay TFC (1996). Introduction to Quantitative Genetics. 4th Edn, Pearson Education Limited, pp 335-354.

Feng T, Zhao Y, Chu MX, Di R, Zhang Y and Fang L (2009). Bone morphogenetic protein 15 as a candidate gene for prolificacy of Jining Grey Goat.  Chinese Journal of Animal and Veterinary Sciences 40(4):468-475.

Food and Agriculture Organization (1982). Cited from

Galloway SM, McNatty KP, Cambridge LM, Laitinen MP, Juengel JL, Jokiranta TS, McLaren RJ, Luiro K, Dodds KG, Montgomery GW, Beattie AE, Davis GH and Ritvos O (2000). Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nature Genetics 25:279-283.

Ghaffari M (2007). Detection of polymorphism in BMPR-IB gene associated with twining in Shal sheep using PCR-RFLP method. International Journal of Agriculture and Biology 11(1):9799.

Hanrahan P, Gregan S, Mulsant P, Mullen M, Davis G, Powell R and Galloway S (2004). Mutations in the genes for oocyte derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biology of Reproduction 70:900-909.

Heikal HSM and Nabi WSH (2017). Genetic Improvement of Litter Size in Four Goat Breeds in Egypt using Polymorphism in Bone Morphogenetic Protein 15 Gene. Advances in Animal and Veterinary Sciences 5(10):410-415.

Hua GH, Chen SL, Ai JT and Yang LG (2008). None of the polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science 108:279-86.

Jalbani MA, Kaleri HA, Baloch AH, Bangulzai N, Bugti AG, Ashraf F, Kaleri RR, Jan M, Bugti GA  and Khosa AN (2017). Study of BMP15 gene Polymorphism in Lehri Goat Breed of Balochistan. Journal of Applied Environmental and Biological Sciences 7(2):84-89. Jamshidi R, Kasirian MM and Rahimi GA (2013). Application of PCR-RFLP technique to determine Booroola gene polymorphism in the Sangsari sheep breed of Iran. Turkish Journal of Veterinary and Animal Sciences 37:129-133.

Kasiriyan MM, Hafezeyen H, Sayahzadeh S, Jamshidi R, Asghari SR, Irajeyan GH and Buesagh H (2009). Genetic polymorphism of FecB and BMP15 genes and its association with litter size in Sangsari sheep breed of Iran. Journal of Animal and Veterinary Advances 8(5):1025-1031.

Kumar S, Mishra AK, Kolte AP, Arora AL, Singh D and Singh VK (2008). Effects of the Booroola (FecB) genotype on growth performance, ewe’s productivity efficiency and litter size in Garole x Malpura sheep. Animal Reproduction Science 105(3-4):319-31.

Kumari R, Dayal S, Kumar S, Lal SV, Chakraborti A, Barari SK and Dey A (2015). Genetic polymorphism of bone morphogenetic protein receptor type-1 gene in Black Bengal goat and its association with litter size. Indian Journal of Animal Sciences 85(5):469-471.

Lawar VS, Deokar DK, Andhale RR and Pawar BK (2008). Management practices adopted by Osmanabadi goat owners in its breeding tract. Journal of Maharashtra Agricultural Universities 33(3):368-372.

Lin J, Du Z, Qin C, Wang J and Ran X (2007). Polymorphism of BMP15 gene in Guizhou white goats. Animal Husbandry and Veterinary Medicine 39(12):21-24.

Michailidis M, Avdi M and Pappa V (2008). Reproductive performance and investigation of BMPR-IB and BMP-15 gene mutations in Greek Chios and Florina sheep breeds. Archiva Zoo Technical 11(1):24-31.

Nawaz A, Babar ME, Hussain T, Nadeem A, Bilal F, Javed K and K Muhammad (2013). Identification of Molecular Markers in BMP15 gene of Pakistani Goat breeds. International Journal of Advanced Research 1:62-68.

Polley S, De S, Batabyal S, Kaushik R, Yadav P, Arora JS, Chattopadhyay S, Pan S, Datta TK and Goswami SL (2009). Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Ruminant Research 35901-35908.

Roy J, Polley S, De S, Mukherjee A, Batabyal S, Pan S, Brahma B, Datta TK and Goswami SL (2011). Polymorphism of fecundity genes (FecB, FecX, and FecG) in the Indian Bonpala sheep. Animal Biotechnology 22(3):151-162.

Sambrook JF and Russell DW (2001). Molecular Cloning: A Laboratory Manual. 3rd Edn, Cold Spring Harbor Laboratory Press USA.

Sharma LSV, Kuralkar S, Ali S, Bankar PS and Kuralkar P (2016). Polymorphism study of BMPR-1B and BMP-15 genes in Berari goats. Indian Journal of Small Ruminants 22(2):230-233

Shokrollahi B (2015). Investigation of BMP15 gene polymorphisms associated with twining in Markhoj goat. Biharean Biologist 9(1):1-4

Souza CJ, MacDougall C, MacDougall C, Campbell BK, McNeilly AS and Baird DT (2001). The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene. Journal of Endocrinology 169 (2):R1-R6

Supakorn C and Pralomkarn W (2010). Sheep FecB gene polymorphism role in Thai meat goat proliferation rate. In: Proceedings of 9th World Congress Genetics Applied to Livestock Production.

Tajangookeh, HD, Shahneh AZ, Zamiri MJ, Daliri M, Kohram H and Javaremi AN (2009). Study of BMP15 gene polymorphism in Iranian goats. African Journal of Biotechnology 8(13):2929-2932.

Wang YQ, Li YX, Zhang N, Wang ZB and Bai JY (2011). Polymorphism of exon 2 of BMP15 gene and its relationship with litter size of two Chinese goats. Asian-Australasian Journal Animal Science 24(7):905-911.