Genotyping of Osmanabadi goat at FecX and FecB LOCI

Genotyping of Osmanabadi goat at FecX and FecB LOCI

Title: Genotyping of Osmanabadi goat at FecX and FecB LOCI

Authors: KG Bhave, MP Sawane, PT Wagh, KA Zunjarrao, VD Pawar and AY Doiphode

Source: Ruminant Science (2017)-6(2):207-214.

Cite this reference as: Bhave KG, Sawane MP, Wagh PT, Zunjarrao KA, Pawar VD and Doiphode AY (2017). Genotyping of Osmanabadi goat at FecX and FecB LOCI. Ruminant Science 6(2):207-214.

Abstract

Prolificacy is one of the most important economical traits of goat. It is affected by various genetic and non-genetic parameters in small ruminants. We have carried out genotyping of Osmanabadi goats of either sex (n=160, 152 females and 08 males) at FecX loci (FecXI, FecXH, FecXB, FecXG and FecXL) of the BMP15 gene with T-ARMS-PCR. FecB/ Booroola locus of BMPR1B gene was, however genotyped using AvaII PCR-RFLP method. T-ARMS-PCR products of FecX loci (FecXI, FecXH and FecXL) and PCR-RFLP of FecB locus exhibited wild type alleles. FecXB and FecXG loci could amplify only control fragment, were subjected for direct sequencing of the control fragment. Sequencing revealed wild type alleles at nucleotide position 213 (G) of FecXB and at position 86 (C) FecXG locus. PCR-RFLP of FecB PCR products revealed a single band of 190 bp representing normal non-carrier animals. It indicated lack of FecX (BMP15) and FecB/ Booroola (BMPR1B) polymorphism in tested Osmanabadi goats. The prolificacy in Osmanabadi goats is therefore found to be independent of these established ovine fecundity gene polymorphisms.

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