IDENTIFICATION OF LIVER CYTOCHROME ENZYMES INVOLVEMENT IN LINCOMYCIN METABOLISM IN GOAT
Title: IDENTIFICATION OF LIVER CYTOCHROME ENZYMES INVOLVEMENT IN LINCOMYCIN METABOLISM IN GOAT
Authors: V Manoja, MK Lonare, Chandra Bhadra, R Kaur and SPS Saini
Source: Ruminant Science (2018)-7(2):217-214.
Cite this reference as: Manoja V, Lonare MK, Bhadra Chandra, Kaur R and Saini SPS (2018). Identification of liver cytochrome enzymes involvement in lincomycin metabolism in goat. Ruminant Science 7(2):217-214.
Present study was planned to see the involvement of liver CYP isoenzymes in lincomycin metabolism in goats and kinetic analysis under in vitro system. Liver S9 fraction was prepared and in-vitro metabolism-kinetics of lincomycin was performed by adding lincomycin (50 µg.ml-1). The total protein and cytochrome P450 content was estimated 23.74± 0.9 mg/g of wet tissue and 0.56±0.10 nmol/mg of protein, respectively. Exposure of licomycin with S9 fraction of goat caused a decrease in lincomycin concentrations in time dependant manner. Kinetic parameter such as Km and Vmax was found to be 27.02 µg and 0.18 nM/min/mg of protein, respectively. Other parameters, in vitro elimination half life (t1/2)and intrinsic clearance (CLint) of was measured as 46.47±7.23 min and 5.68±0.91 mL/min/g, respectively. 65% of lincomycin was metabolized within 1 hour by S9 fraction. Drug metabolism inhibition study showed that participation CYP 1A2, 3A4, 2C9 and 2D6 enzymes in reaction do not alters the rate of lincomycin metabolism. Present study concluded that the tested hepatic liver isoenzymes do not play any role in licomycin metabolism in goats.
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