Identification of SNP in growth hormone gene and their association

Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep

Title: Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep

Authors: Vaishali Hiremath, MM Appannavar, Yathish HM, Sangamesh, SM Kartikesh, Vivek R Kasaralikar and Siddalingaswamy Hiremath

Source: Ruminant Science (2017)-6(2):221-224.

Cite this reference as: Hiremath Vaishali, Appannavar MM, HM Yathish, Sangamesh, Kartikesh SM, Kasaralikar VR and Hiremath Siddalingaswamy (2017). Identification of SNP in growth hormone gene and their association with growth traits in Kenguri sheep. Ruminant Science 6(2):221-224.

Abstract

The research was performed with an aim of identifying SNPs in growth hormone (GH) gene using PCR-RFLP analysis and to associate them with various growth traits in Kenguri sheep. For this, a fragment of 422bp spanning between exon 2 and 3 of GH gene was amplified from 60 Kenguri sheep. GH/ HaeIII RFLP analysis of these fragments for locus A781G has showed only AA (with 366 bp and 56bp bands) and AB (with 422bp, 366bp and 56 bp bands) genotypes and BB genotype was absent in the studied sheep population. The respective frequency of AA, and AB genotype was 0.42 and 0.58 and that of A and B allele was 0.71 and 0.29. Chi-square analysis of GH/HaeIII polymorphism showed noncompliance of Hardy Weinberg equilibrium in studied Kenguri population. The Mean±SE values for body weight (kg), height at wither (cm), body length (cm) and chest girth (cm) were 34.98±0.59, 70.97±0.375, 69.22±0.50 and 79.72±0.72 in ewes, while in rams the respective values were 37.46±0.69, 71.25±0.59, 69.18±0.90 and 83.18±0.84. Analysis of these data using ‘t’ test revealed a significant (p<0.05) difference between ram and ewe with respect to body weight and chest girth only. Association analysis revealed non-significant difference between AA and AB genotypes for body weight, height at wither, body length and chest girth both in rams and ewes of Kenguri sheep. The non correlation between different genotypes and growth traits in this investigation may be accounted to small sample size. However, the association may be established if the sample size is large and in turn it can be used for selection of animals for breeding to bring improvement in mutton production.

References

Ahlawat AR, Gajbhiye PU, Prince LLL, Meena AS, Dongre VB and Gajjar SG (2014). Genetic polymorphisms in growth hormone gene in Patanwadi, Marwari and Dumba breeds of sheep. Indian Journal of Animal Sciences 84(9):1021-22

Ayuk J and Sheppard MC (2006). GH and its disorders. Postgraduate Medical Journal 82:24-30

Hajihosseinlo A and Negahdary M (2013). Single nucleotide polymorphism in growth hormone gene exon-4 using PCR-SSCP in Makooei Sheep. Journal of Biology and Today’s World 2322-3308.

Hua GH, Chen SL, Yu JN, Cai KL, Wu CJ, Li QL, Zhang CY, Liang AX, Han L, Geng LY, Shen Z, Xu DQ and Yang LG (2009). Polymorphism of growth hormone gene and its association with growth traits in Boer goat bucks. Meat Science 81:391.

Jain A, Kulkarni VS, Govindaiah MG, Sadana DK, Aswathnarayan T, Pandey AK, Kumar D, Sharma R and Ahlawat SPS (2006). Sheep Genetic Resources of India. Kenguri Monograph-33, 2006 NBAGR, Karnal, India.

Jain A, Kulkarni VS, Govindaiah MG, Sadana DK, Aswathnarayan T, Pandey AK, Kumar D, Sharma R and Singh G (2005a). Sheep Genetic Resources of India: Bellary Monograph-2005. National Bureau of Animal Genetic Resources, Karnal, India.

Jain A, Sadana DK, Govindaiah MG, Kulkarni VS, Aswathnarayan T, Pandey AK, Kumar D, Sharma R and Singh G (2005b). Sheep Genetic Resources of India. Mandya Monograph- 2005. National Bureau of Animal Genetic Resources, Karnal, India.

Jain Anand, Yadav DK, Kulkarni VS, Govindaiah MG, Aswathnarayan T and Sadana DK (2014). A comparison of morphometric traits of sheep breeds of Karnataka in the farmers’ flocks. Indian Journal of Animal Sciences 84(8):916-918

Miller SA, Dykes D and Polesky HF (1988). A sample salting out procedure for extraction of DNA from human nucleated cells. Nucleic Acids Research 16(3):1215

Kumari R, Kumar R, Meena AS, Jyotsana B, Prince LLL and Kumar S (2014a). Genetic polymorphism of growth hormone gene in native sheep breeds of India. Indian Journal of Small Ruminants 20(2):15-18

Kumari R, Prince LLL, Kumar R, Meena AS, Jyotsana B and Prakash V (2014b). Effect of gene polymorphism of growth hormone on growth traits in Malpura and Avikalin sheep. Indian Journal of Small Ruminants  20(2):106-108.

Sangamesh, Appannavar MM, Yathish HM, Hiremath V, Kulkarni S, Ramachandra B and Suranagi MD (2017). Variation in growth hormone gene and its association with growth traits in Osmanabadi goat. Ruminant Science 6(1):79-83.

Seevagan M, Jeichitra V, Rajendran R and Tirumurugaan KG (2015). Detection of lethal SNP (A781G) in growth hormone (GH) gene of Indian sheep. Small Ruminant Research 126:13-15.

Snedecor GW and Cochran WG (1994). Statistical Methods. Iowa state University Press, Ames, Iowa

Tahmoorespur M, Taheri A, Gholami H and Ansary M (2011). PCR-SSCP variation of GH and STAT5A genes and their association with estimated breeding values of growth traits in Baluchi sheep. Animal Biotechnology 22(1):37-43.