In vitro study of dietary supplementation of Malva sylvestris

In vitro study of dietary supplementation of Malva sylvestris to Suaeda fruticosa plant on rumen digestibility, fermentation and protozoa morphology in one-humped camel

Title: In vitro study of dietary supplementation of Malva sylvestris to Suaeda fruticosa plant on rumen digestibility, fermentation and protozoa morphology in one-humped camel

Authors: Tahereh Mohammadabadi and Morteza Chaji

Source: Ruminant Science (2019)-8(1):1-8

Cite this reference as: Mohammadabadi Tahereh and Chaji Morteza (2019). In vitro study of dietary supplementation of Malva sylvestris to Suaeda fruticosa plant on rumen digestibility, fermentation and protozoa morphology in one-humped camel. Ruminant Science 8(1):1-8.

Abstract

This experiment aimed to investigate the supplementary effect of 0, 20, 40 and 60 mg/kg Malva sylvestris on protozoa species, digestibility and microbial fermentation of Suaeda fruticosa plant in the rumen liquor of one-humped camel. Fermentation parameters were measured by gas production technique and in vitro digestibility by two-stage digestion using rumen liquor from two female fistulated dromedary camels. Protozoa species was studied in vitro gas production condition. The results showed that addition of Malva sylvestris linearly increased gas production potential and rate constant from Suaeda fruticosa forage in camels (P<0.05). Addition of Malva sylvestris at 60 mg/kg dry matter to Suaeda fruticosa, significantly decreased partitioning factor, microbial biomass and the efficiency of microbial biomass (P<0.05), while organic matter digestibility linearly increased, but had no influence on cell wall degradability (P>0.05). Treatments containing Malva sylvestris (40 and 60 mg) significantly reduced ammonia-nitrogen linearly (P<0.05) but didn’t influence pH and total protozoa population (P>0.05). Digestibility of DM (dry matter) and NDF (neutral detergent fibre) and species of Diplodinium cameli in treatments containing Malva sylvestris were the highest but species of Diplodinium ecaudatum decreased in camels (P<0.05). Therefore, the results indicated that addition of Malva sylvestris improved digestibility and gas production with a decrease in ruminal ammonia-N concentration; that Malva sylvestris supplementation can be used to improve the nutritional value of Suaeda fruticosa forage in dromedary camel.

References

Ahwazi M, Rezvani A and Habibi Khanani B (2010). Seeds of medicinal plants (morphology, physiology and medicinal properties). Tehran University Press. 131-202.

Alexander G, Singh B, Sahoo A and Bhat TK (2008). In vitro screening of plant extracts to enhance the efficiency of utilization of energy and nitrogenin ruminant diets. Animal of Feed Science and Technology 145:229-242.

Alipour D (2012). Ciliatea protozoa in Sindi and Baluchi camels. Journal of Veterinary Research 67(3):263-257.

Allen MS (1997). Relationship between ruminal fermentation and requirement for physically effective fiber. Journal of Dairy Science 80:1447-1462.

AOAC (2000). Association of Official Analytical Chemists Official Methods of Analysis Washington DC, USA.

Baah J, Ivan M, Hristov AN, Koenig KM, Rode LM and McAllister TA (2007). Effects of potential dietary antiprotozoal supplements on rumen fermentation and digestibility in heifers. Animal Feed Science and Technology 137:126-137.

Barros L, Carvalho AM and Ferreira ICFR (2009). Leaves, flowers, immature fruits and leafy flowered stems of Malva sylvestris: A comparative study of the nutraceutical potential and composition. Portugal, Food and Chemical Toxicology 47:2458-2464.

Blummel M, Makkar HPS and Becker K (1997). In vitro gas production – A technique revisited. Journal of Animal Physiology and Animal Nutrition 77:24-34.

Bodas R, Fernández M, García-González R, González JS, López S and Wallace RJ (2009). Phytogenic additives to decrease in vitro ruminal methanogenesis. Options Méditerranéennes. Série A, Séminaires Méditerranéens N  85:279-283.

Bonhomme A (1990). Rumen ciliates: their metabolism and relationship whit bacteria and their hosts. Animal Feed Science and Technology 30:203-266.

Burt S (2004). Essential oils: their antibacterial properties and potential applications in foods a review. International Journal of Food Microbiology 94:223-253.

Dehority BA (2003). Rumen microbiology. British Library Cataloguing in Publication Data. First Published.

Dong GZ, Wang XJ, Liu ZB and Wang F (2010). Effects of phytogenic products on in vitro rumen fermentation and methane emission in goats. Journal of Animal and Feed Sciences 19:218-229.

Emanuele SM, Staples CR and Wilcox CJ (1991). Extent and site of mineral release from six forage species incubated in mobile dacron bags. Journal of Animal Science 69:801-810.

Francis G, Kerem Z, Makkar HPS and Becker K (2002). The biological action of saponins in animal systems: A review. British Journal of Nutrition 88:587-605.

Ghali MB, Scott PT and Al Jassim RAM (2005). Effect of diet change on population of rumen protozoa in dromedary camel. Recent Advances in Animal Nutrition in Australia 15.

Hobson PN and Stewart CS (1997). The rumen microbial ecosystem. Elsevier Science Publishers Ltd, London and New York.

Hristov AN, Ivan M and McAllister TA (2004). In vitro effects of individual fatty acids on protozoal numbers and on fermentation products in ruminal fluid from cattle fed a high-concentrate, barley-based diet. Journal of Animal Science 82:2693-2704.

Khan US and Bano A (2011). Physiological and biochemical analysis of the selected halophytes of district Mardan, Pakistan. International Journal of Bioscience, Biochemistry and Bioinformatics 1(4).

Lila ZA, Mohammed N, Kanda S, Kamada T and Itabashi H (2003). Effect of sarsaponin on rumen fermentation with particular reference to methane production in vitro. Journal of Dairy Science 86:3330-3336.

Makkar HPS and Becker K (1998). Do tannins in leaves of trees and shrubs from African and Himalayan regions differ in level and activity? Agroforestry System 40:59-68.þ

Maldar SM, Roozbehan Y and AliPour D (2010). The effect of adaptation period to oak leaves on digestibility and rumen parameters of Alamut goats, Iranian Journal of Animal Science, 41(43):243-252.

McSweeney CS, Palmer B, McNeill DM and Krause DO (2001). Microbial interactions with tannins: nutritional consequences for ruminants. Animal Feed Science Technology 91:83-93.

Menk KH and Stingass H (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28:6-55.

Min BR, Attwood GT, Reilly K, Sun W, Peters JS, Barry TN and McNabb WC (2002). Lotus corniculatus condensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep. Canadian Journal of Microbiology 48:911-921.

Ogimoto K and Imai S (1981). Atlas of rumen microbiology. Japan Scientific Societies Press, Tokyo.

Ørskov ER and McDonald I (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of Agricultural Science 92:499-503.

Patra AK and Saxena K (2010). A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry 5-10.

Sallam SMA, Bueno ICS, Brigide P, Godoy PB, Vitti DMSS and Abdalla AL (2009). Investigation of potential new opportunities for plant extracts on rumen microbial fermentation in vitro. Options Mediterraneennes: Serie A, Seminaires Mediterraneens 85:255-260.

Sallam SMA, da Silva Bueno IC, de Godoy PB, Eduardo FN, Schmidt Vittib DMS and Abdalla AL (2010). Ruminal fermentation and tannins bioactivity of some browses using a semi-automated gas production technique. Tropical and Subtropical Agroecosystems 12:1-10.

Sliwinski BJ, Soliva CR, Machmuller A and Kreuzer M (2002). Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Animal of Feed Science and Technology 101:101-114.

Sommart K, Parker DS, Rowlinson P and Wanapat M (2000). Fermentation characteristics and microbial protein synthesis in an in vitro system using cassava, rice straw and dried ruzi grass as substrates. Asian-Aust Journal Animal Science 13:1084-1093.

Tabaraki R, Yosefi Z and Asadi Gharneh HA (2012). Chemical Composition and Antioxidant Properties of Malva sylvestris L, Journal of Research in Agricultural Science 8(1):59-68.

Tilly JMA and Terry RA (1963). A two stage technique for the indigestion of forage crops. Journal of British Grassland Society 18:104-111.

Towhidi A and Zandi M (2007). Chemical composition in vitro digestibility and palatability of nine plant species for dromedary camels in the province of Semnan, Iran. Egyptian Journal of Biology 9:47-52.

Van Soest PJ, Robertson JB and Lewis BA (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74:3583.

Wina E, Muetzel S and Becker K (2005). the impact of saponins or saponin-containing plant materials on ruminant productions: A review. Journal of Agriculture and Food Chemical 53:8093-8105.