Molecular and patho-biochemical characterization of Mycobacterium tuberculosis infection in cow

 Molecular and patho-biochemical characterization of Mycobacterium tuberculosis infection in cow

Title: Molecular and patho-biochemical characterization of Mycobacterium tuberculosis infection in cow

Authors: Rajat Varshney, R Singh, B Sonkar, S Singh, A Barnwal, SS Choudhary, R Verma and A Khatti

Source: Ruminant Science (2017)-6(2):315-318.

Cite this reference as: Varshney Rajat, Singh R, Sonkar B, Singh S, Barnwal A, Choudhary SS, Verma R and Khatti A (2017). Molecular and patho-biochemical characterization of Mycobacterium tuberculosis infection in cow. Ruminant Science 6(2):315-318.


A case of 6-year-old died cow of military dairy farm was submitted to the Division of Pathology, IVRI for necropsy examination. Grossly, variable sized tubercles were present on vital organs as well as the classical histopathological lesions strongly suggested the case of bovine tuberculosis.  Furthermore, the bacterial isolation, various biochemical tests and a 12.7 kb fragment based multiplex PCR confirmed the involvement of Mycobacterium tuberculosis in cow.   


Ameni G, Aseffa A, Engers H, Young D, Gordon S, Hewinson G and Vordermeier M (2007). High prevalence and increased severity of pathology of bovine tuberculosis in Holsteins compared to zebu breeds under field cattle husbandry in central Ethiopia. Clinical and Vaccine Immunology 14(10):1356-61.

Bakshi CS, Shaha DH, Verma R, Singh RK and Malik Meenakshi (2005). Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR. Veterinary Microbiology 109(3-4):211-216.

Cardoso S, Martin A, Mejia G, Palomino J, Da Silva M and Portaels F (2004). Practical handbook for the phenotypic and genotypic identification of mycobacteria. Section 1.

Cassidy JP, Bryson DG, Pollock JM, Evans RT, Forster F and Neill SD (1999). Lesions in cattle exposed to Mycobacterium bovis-inoculated calves. Journal of Comparative Pathology 121(4):321-37.

Chandrasekharan KP and Ramakrishnan R (1969). Bovine tuberculosis due to the human strain of Mycobacterium tuberculosis. Indian Journal of Tuberculosis 16:103-105.

Corner LA (1994). Post mortem diagnosis of Mycobacterium bovis infection in cattle.    Veterinary Microbiology 40(1-2):53-63.

 De la Rua-Domenech R, Goodchild AT, Vordermeier HM, Hewinson RG, Christiansen KH and Clifton-Hadley RS (2006). Ante mortem diagnosis of tuberculosis in cattle: A review of the tuberculin tests, alpha-interferon assay and other ancillary diagnostic techniques. Research in Veterinary Science 81(2):190-210.

International Office of Epizootics (2008). Biological Standards Commission, International Office of Epizootics. International Committee. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Mammals, birds and bees. Office international des epizooties.

Mondal T (2014). Quantitative real time PCR based IFN-alpha assay for diagnosis of bovine tuberculosis using expressed recombinant CFP-10. PhD thesis submitted to the Deemed University Indian Veterinary Research Institute, Izatnagar, UP, India

Palomino JC, Leão SC and Ritacco V (2007). Tuberculosis 2007. 1st Edn,

Shah DH, Verma R, Bakshi CS and Singh RK (2002). A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. FEMS Microbiology Letters 214(1):39-43.

Steele JH (1980). Human tuberculosis in animals. In: CRC Handbook Series in Zoonoses. Section-A. Bacterial, Rickettsial and Mycotic diseases. Eds: JH Steele. Vol 2. CRC Press, Inc., Boca Raton, Fla. pp 141-159.

Thoen CO, Karlson AG and Himes EM (1981). Mycobacterial infections in animals. Reviews of Infectious Diseases 3:960-972.

World Health Organization (2011). Global tuberculosis control: WHO Report.