Prevalence of toxigenic Escherichia coli (E.Coli)

Prevalence of toxigenic Escherichia coli (E.Coli) and rotavirus among neonatal calves in south Gurarat region (India) using classical and PCR based methods

Title: Prevalence of toxigenic Escherichia coli (E.Coli) and rotavirus among neonatal calves in south Gurarat region (India) using classical and PCR based methods

Authors: Sanjay G Malaviya, IH Kalyani, KK Sharma, PS Sakhare and DR Patel

Source: Ruminant Science (2017)-6(1):139-144.

Cite this reference as: Malaviya Sanjay G, Kalyani IH, Sharma KK, Sakhare PS and Patel DR (2017). Prevalence of toxigenic Escherichia coli (E.Coli) and rotavirus among neonatal calves in south Gurarat region (India) using classical and PCR based methods. Ruminant Science 6(1):139-144.

Abstract

A total of 62 faecal samples were collected from diarrhoeic bovine calves aging upto 8 weeks of age and processed for presence of rotavirus and E.coli. The enteropathogens detected were E. coli in 45/62(72.58%) samples via bacteriological and molecular methods and rotavirus in 7/62 (11.29%) samples via Latex agglutination test (LAT), respectively and their co- presence were found in 5/62 (8.06%) cases. All the LAT positive rotavirus samples were though found negative by RT-PCR. Among the 25 samples sent for serotyping, ten isolates were serotyped as O25, O136 (04 isolates each), O1 and O34 (01 isolate each), while 15 isolates could not be serotyped with available antisera. PCR based detection of bacteria confirmed the presence of E. coli. PCR of toxigenic genes revealed absence of heat labile (LT) and heat stable (ST) toxins but 4 isolates (8.89%) were found positive for verotoxin (VT) genes. where one isolate (2.22%) possessed VT1 gene and 3 isolates (6.67%) VT2 gene. When E.coli isolates were subjected to antibiotic sensitivity test, Enrofloxacin, Gatifloxacin and Trimethoprim were found most effective antibiotics and respectively, 4.44 %, 11.11 % and 13.33 % isolates showed resistant against them. On the other hand maximum resistance was shown against Cephalexin (93.33%) and Kanamycin (88.89%). Conclusively, verotoxigenic E.coli was detected as a major enteropathogen of neonatal calf diarrhoea, along with rotavirus and fluroquinoles group of antibiotics were found as effective antibacterial drugs.

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