10-Title: RELATIVE EXPRESSION OF OOCYTE COMPETENCE MARKERS AS BIOMARKERS OF OOCYTE QUALITY IN GOATS
Authors: Abhiyuday Pratap Singh, GK Mishra, Sriti Pandey, Abhiskek Asati, Gaytri Devi, Mayank Sharma, Deepak Ningwal, Kashmi Sharma, Megha K Pandey, SK Pandey, Neeraj Srivastava and Ajit Pratap Singh
Source: Ruminant Science (2025)-14(1):57-62.

Abstract

10. How to cite this manuscript: Singh Abhiyuday Pratap, Mishra GK, Pandey Sriti, Asati Abhiskek, Devi Gaytri, Sharma Mayank, Ningwal Deepak, Sharma Kashmi, Pandey Megha K, Pandey SK, Srivastava Neeraj and Singh Ajit Pratap (2025). Relative expression of oocyte competence markers as biomarkers of oocyte quality in goats. Ruminant Science 14(1):57-62.
Abstract
The present study was designed to determine the relative expression of growth differentiation factor 9 (GDF-9), hyaluronan-synthase-2 (HAS-2) and prostagladin-endoperoxide synthase-2 (PTGS-2) in good (Grade I and II) and poor (Grade III and IV) quality oocytes cumulus cells as biomarkers of oocyte quality. A total 144 caprine ovaries were collected from local slaughterhouse. A total of 391, 344, 218 and 146 oocytes of grades I, II, III and IV, respectively, were recovered by aspiration method with the respective recovery rates of 35.58%, 31.30%, 19.83% and 13.29%. Total RNA was extracted from the oocytes and cumulus cells and real time PCR amplification of GDF-9, HAS-2 and PTGS-2 was done using â-actin and GAPDH as endogenous controls. The poor quality oocytes and CCs were kept as a calibrator group. The relative fold changes of GDF-9 (2.15 folds) in good quality oocytes were significantly (p<0.01) up-regulated as compared to the poor quality oocytes. 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