10-Title: INCIDENCE AND DIAGNOSTIC EFFICACY OF FAECAL AND TISSUE SMEAR EXAMINATION FOR CAPRINE PARATUBERCULOSIS IN GOATS OF MHOW REGION
Authors: Shivraj Chouhan, Gaya Prasad Jatav, Joycee Jogi, Supriya Shukla, Nidhi Shrivastava, AK Jayraw, M Shakya, Akash Suman and Supnesh Jain
Source: Ruminant Science (2025)-14(2):159-164.

Abstract

How to cite this manuscript: Chouhan Shivraj, Jatav Gaya Prasad, Jogi Joycee, Shukla Supriya, Shrivastava Nidhi, Jayraw AK, Shakya M, Suman Akash and Jain Supnesh (2025). Incidence and diagnostic efficacy of faecal and tissue smear examination for caprine paratuberculosis in goats of Mhow region. Ruminant Science 14(2):159-164.
Abstract
The present study aimed to assess the prevalence of paratuberculosis in 250 goats presented for slaughter at the Mhow slaughterhouse, Madhya Pradesh, between November 2023 and April 2024. The overall incidence of paratuberculosis, as determined by faecal smear examination, was 30.80% (77/250). Tissue impression smear analysis revealed Mycobacterium avium subsp. paratuberculosis (MAP) positivity in 19.20% (48/250) of intestinal samples and 15.60% (39/250) of mesenteric lymph node samples. Gross pathological examination demonstrated organ-specific lesions characteristic of chronic MAP infection, including markedly thickened intestinal walls and enlarged, cord-like mesenteric lymph nodes. Faecal microscopy proved to be a relatively sensitive diagnostic method, particularly in clinically affected animals. Body condition scoring indicated that although 38.40% of the goats appeared clinically healthy, a substantial proportion of MAP-positive animals fell within this category, highlighting the presence of subclinical infections. The highest incidence of paratuberculosis was observed in goats older than two years (19.60%) and in females (21.20%), suggesting increased susceptibility associated with age and sex. Impression smear examination frequently revealed acid-fast bacilli within intestinal macrophages, further supporting a diagnosis of chronic paratuberculosis. Collectively, these findings demonstrate that faecal microscopy and tissue impression smear techniques are reliable and practical diagnostic tools for detecting MAP infection in endemic regions. The study underscores the considerable prevalence of paratuberculosis among goats in Central India and emphasizes the urgent need for strengthened diagnostic surveillance and effective control strategies to mitigate the disease in small ruminant populations.
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