1-Title: Ruminant mammary stem cells: Methods of identification and status

Authors: Ratan K Choudhary and Shanti Choudhary

Source: Ruminant Science (2012)-1(2):101-108.

How to cite this manuscript: Choudhary RK and Choudhary Shanti (2012). Ruminant mammary stem cells: Methods of identification and status. Ruminant Science 1(2):101-108.


Mammary stem cells (MaSC) are the epithelial stem cell, which generates functional mammary gland upon transplantation into epithelium-cleared mammary fat pad. Two distinct functions of MaSC are; 1) maintenance of stem cell population (self-renewal) and, 2) production of differentiated cells of various cell lineages (differentiation). Knowledge of MaSC will provide insight into extensive expansion capacity and differentiation characteristics of mammary gland. Additionally, identification of cell hierarchy will broaden understanding of mammary gland biology. In this review, we have discussed different methods of identification of human and mouse MaSC. These methods were employed to isolate bovine and goat MaSC. We have also discussed the status of ruminant (bovine, goat, sheep and buffalo) MaSC. Knowledge of bovine, goat and sheep MaSC is recent and limited whereas information on buffalo MaSC is still lacking.


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