10-Title: Serotyping and in vitro drug sensitivity of Escherichia Coli isolated from neonatal buffalo calves with diarrhoea
Authors: DK Kapadiya, DV Joshi, NM Shah and HH Panchasara
Source: Ruminant Science (2012)-1(2):141-143.
How to cite this manuscript: Kapadiya DK, Joshi DV, Shah NM and Panchasara HH (2012). Serotyping and in vitro drug sensitivity of Escherichia Coli isolated from neonatal buffalo calves with diarrhoea. Ruminant Science 1(2):141-143.
Neonatal calf diarrhoea of E. coli origin is one of the leading causes of mortality posing a serious threat to dairy industry. In the present study, a total of 43 diarrhoeic faecal samples were collected from neonatal buffalo calves up to one month of age from an organized farm. Out of 43 faecal samples, E. coli alone was encountered in 34 (79.06%) cases. Out of 34 E. coli isolates, 29 isolates were serotyped which belonged to 17 different serogroups whereas 5 isolates were untypable. The frequently observed serotypes were O22 and O153 (4 isolates each), O132 (3 isolates), O8, O45, O86, O98 and O120 (2 isolates each), and one each of O17, O53, O78, O92, O100, O101, O143 and O156. All the 34 E. coli isolates were found sensitive to gentamicin. The other antibiotics found effective in decreasing order of their efficacy were colistin, 30 (88.24%), furazolidone, 28 (82.35%), enrofloxacin, 20 (58.82%), chloramphenicol, 20 (41.18%) and cotrimoxazole, 9 (26.47%). All E. coli isolates were found resistant to ampicillin and oxytetracycline.
Arya E (2005). Isolation and identification of E. coli from diarrhoeic calf faeces by biochemical tests, antibiogram pattern and PCR based detection of toxigenic genes. MVSc thesis submitted to Anand Agricultural University, Anand.
Bauer AW, Kirby WMM, Sherris JC and Toun M (1966). Antibiotic susceptibility testing using a standard single disc method. American Journal of Clinical Pathology 45:493-496.
Bergey’s Manual of Determinative Bacteriology (1974). 8th Edn. The Williams and Wilkins Company, Baltimore. USA.
Blanco M, Blanco JE, Mora A and Blanco J (1998). Prevalence and characteristics of necrotoxigenic E. coli CNF1+ and CNF2+ in healthy cattle. Research in Microbiology 149(1):47-53.
Chakraborty D and Nag NC (1997). Isolation and characterization of E. coli associated with diarrhoeic cases of cattle and man. Indian Journal of Animal Health 36:147-150.
Chaurasia R and Soni SS (2002). Antibiotic sensitivity and transmissible drug resistance of E. coli associated with calf diarrhoea. Compendium national symposium on recent trends in diagnostics and therapeutics of animal disease and XX Annual Convention of Indian Society for Veterinary Medicine, 14-16th February p 76.
Cruickshank R and Duguid JP, Mormion BP and Swain RHA (1975). Medical Microbiology, 12th Edn Vol 1 and 2 Churchill Livingstone, Margland.
Edwards R and Ewing WH (1972). Identification of Enterobacteriaceae, 3rd Edn Burges Publishing Company, Minnesota.
Gyles CL (1994). In: Escherichia coli in Domestic Animals and Humans. Eds CL Gyles, CAB International Wallingford, UK, pp 365-398.
Himanshu and Pal B (2012). Serotypes of Escherichia coli obtained from neonatal diarrhoeic calves. Indian Veterinary Journal 89(5):89-90.
Joon DS and Kaura YK (1993). Isolation and characterization of some of the enterobacteria from diarrhoeic and non-diarrhoeic calves. Indian Journal of Animal Sciences 63:373-383.
Mehrotra PK, Lakhotia RL and Mehrotra PN (1984). Occurrence of serotypes and antibiotic resistance in E. coli isolates from different sources. Indian Journal of Animal Sciences 54(4):396-399.
Pachaury R and Kataria AK (2012). Serogrouping and colicin-related properties of Escherichia coli isolated from neonate diarrhoeic ruminants. Ruminant Science 1(1):9-13.
Patil RM, Karpe AG and Gujar MB (1999). Studies on drug resistance and virulence of E.coli from diarrhoeic calves. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 20(1):29-31.
Radostits OM, Gay GC, Blood DC and Hinchcliff KW (2000). Veterinary Medicine. 9th Edn, WB Saunders, London p 156.
Saravanbava K, Venugopalan AT and Baluprakasam RA (1990). Enteropathogenic E. coli associated with neonatal calf diarrhea. Cherion 19:29-34.
Shah NM (1989). Microbiological investigation of neonatal calf diarrhoea with specific reference to detection of rotavirus and enterotoxigenic Escherichia coli. PhD thesis submitted to Gujarat Agricultural University, Sardarkrushinagar.
Sharma DK, Soni SS, Kashyap SK and Shringi BN (2004). Seroprevalance, antibiotic sensitivity pattern and transfer of plasmid coded characters of E.coli associated with calf diarrhea. Indian Veterinary Journal 81:6-8.
Sojka WJ (1971). Enteric diseases in newborn piglets, calves and lambs due to Escherichia coli infections. Veterinary Bulletin 41:509-519.