10-Title: Serotyping and in vitro drug sensitivity of Escherichia Coli isolated from neonatal buffalo calves with diarrhoea

Authors: DK Kapadiya, DV Joshi, NM Shah and HH Panchasara

Source: Ruminant Science (2012)-1(2):141-143.

How to cite this manuscript: Kapadiya DK, Joshi DV, Shah NM and Panchasara HH (2012). Serotyping and in vitro drug sensitivity of Escherichia Coli isolated from neonatal buffalo calves with diarrhoea. Ruminant Science 1(2):141-143.


Neonatal calf diarrhoea of E. coli origin is one of the leading causes of mortality posing a serious threat to dairy industry. In the present study, a total of 43 diarrhoeic faecal samples were collected from neonatal buffalo calves up to one month of age from an organized farm. Out of 43 faecal samples, E.  coli alone was encountered in 34 (79.06%) cases. Out of 34 E. coli isolates, 29 isolates were serotyped which belonged to 17 different serogroups whereas 5 isolates were untypable. The frequently observed serotypes were O22 and O153 (4 isolates each), O132 (3 isolates), O8, O45, O86, O98 and O120 (2 isolates each), and one each of O17, O53, O78, O92, O100, O101, O143 and O156. All the 34 E. coli isolates were found sensitive to gentamicin. The other antibiotics found effective in decreasing order of their efficacy were colistin, 30 (88.24%), furazolidone, 28 (82.35%), enrofloxacin, 20 (58.82%), chloramphenicol, 20 (41.18%) and cotrimoxazole, 9 (26.47%). All E. coli isolates were found resistant to ampicillin and oxytetracycline.


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