19-Title: Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle

Authors: Vaibhav Kumar, JS Patel, BR Patel, VK Mevada and AP Raval

Source: Ruminant Science (2012)-1(2):177-180.

How to cite this manuscript: Kumar Vaibhav, Patel JS, Patel BR, Mevada VK and Raval AP (2012). Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle. Ruminant Science 1(2):177-180.


The objective of the present study was to determine the therapeutic efficacy of antibiotics in clinical mastitis of cross bred cattle. Therapeutic efficacy of Ceftriaxone with Tazobactum, Gentamicin and Enrofloxacin and their effect on mastitis and impact of therapy on diagnostic indicators such as modified California mastitis test (CMT) and somatic cell count (SCC) and biochemical composition (Per cent of Fat, solid not fat (SNF), lactose and pH) of milk were studied. Cross bred cattle affected with clinical mastitis were divided into three groups having 12 animals in each group. Group-I was treated with Ceftriaxone with Tazobactum @ 5mg/kg, Group-II was treated with Gentamicin @ 4.0mg/kg and Group-III was treated with Enrofloxacin @ 5mg/kg, for three consecutive days intramuscularly (I/M) along with supportive treatment as Pheniramine Maleate @ 0.5 mg/kg and Meloxicam @ 0.3 mg/kg (I/M). Milk samples of all three groups were collected before treatment (day 0) and thereafter on day 3, 5 post treatment periods for the analysis of CMY, SCC and biochemical composition of milk. Ceftriaxone with Tazobactum was found to be more effective treatment (83.33 per cent) as compared to Gentamicin (58.33 per cent) and Enrofloxacin (41.66 per cent) in clinical mastitis.


Awasthi P and Upadhyay AK (2006). Screening of subclinical mastitis using cow side test and California mastitis test. Indian Veterinary Journal 83(3):275-276.

Bansal BK  (2011). Seminar on Practical Approach to disease diagnosis with emphasis on clean milk production. Department of Clinical Veterinary Medicine from Guru Angad dev Veterinary and Animal Sciences University, Ludhiana-141 004.

Chahar A (2007). Studies on comparative evaluation of various screening tests for detection of subclinical mastitis in cows. Intas Polivet 8(1):208-211.

 Dhanda MR and Sethi MS (1962). Investigation of mastitis in India. ICAR research series no. 35. New Delhi, India.

Goswami  P,  Biswas S and Podder RC (2002). Isolation and antibiogram of bacterial isolates from clinical mastitis. Indian Journal of Animal Health 41(1):52-54.

Grewal KD, Gupta MP and Singh KB (2005). Therapeutic efficacy of gentamicin in clinical cases of mastitis of buffaloes. Indian Veterinary Journal 82(2):123-125.

Gruet P, Maincent P, Berthelot X and Kaltsatos V (2001). Bovine mastitis and intramammary drug delivery: Review and perspectives. Advanced Drug Delivery Reviews 50:245-259.

Mukherjee  R and Dash PK (2003). Status of subclinical bovine mastitis in lactating cows of a livestock production research farm. Indian Journal of Animal Science 73(7):775-777.

Paape MJ, Duenas MI, Wettemann RP and Douglass LW (2000). Effects of intramammary infection and parity on calf weaning weight and milk quality in beef cows. Journal of Animal Science 78(10):2508-2514.

Radostitis OM, Gay CC, HInchliff KW and Constable PD (2009). Disease of mammary gland. In: Veterinary Medicine, 10th Edn, Saunders Elsvier, pp 673-762.

Rajsukhbirsingh BK, Bansal BK, Randhawa SS and Mavi PS (2004). Effect of lactation therapy on quarter infection and milk composition in specific mastitis of buffaloes. Indian Journal of Veterinary Medicine 24(1):16-18.

Sadaker RD, Wadhmare SP, Imran K and Doiphode VL (2000). Efficacy of ‘COBACTAN LC’ in clinical mastitis in cows and buffaloes with reference to effect on biochemical profile of milk. Indian Veterinary Journal, 77(10):867-869.

Saluja PS, Gupta SL, Kapur MP and Sharma A (2005). Efficacy of Enrofloxacin alone or with immunomodulators as therapy for mastitis in lactating cows. Indian Veterinary Journal 82(2):150-152.

Schalm OW, Carroll G and Jain NC (1971). Bovine Mastitis. Lea and Fibiger, Philadelphia, USA p 360.

Sharma VK and Rajani HB (1969). California mastitis test. Indian Veterinary Journal 6:749-752.

Snedecor GW and Cochran R (1994). In: Statistical Methods. Oxford and IBH Publishing Co, Calcutta, p 23.

Sudhan NA, Singh R, Singh M and Soodan JS (2010). Studies on prevalence, etiology and diagnosis of sub clinical mastitis among cross bred cows. Indian Journal of Animal Research 39(2):127-130.

Thomas R, George SK, Harshan HM and Antony P (2006). Etiology and antibiogram of bacterial isolates from field cases of mastitis in cows from Kottayam district, Kerala. Veterinary World 4(12):357-360.