2-Title: Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions

Authors: Gopal Puri and Sadhan Bag

Source: Ruminant Science (2012)-1(2):109-112.

How to cite this manuscript: Puri Gopal and Bag Sadhan (2012).Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions. Ruminant Science 1(2):109-112.


The present experiment was carried out to study the effects of feeder or feeder free culture system on developmental potency of in vitro derived buffalo embryos to embryonic stem cell clones. In vitro produced buffalo embryos were made zona free and cultured on two types of feeder layer (buffalo and murine fetal fibroblast monolayer) and two types of feeder free coated plates (matrigel and fibronectin extra cellular matrix) culture system. The percentage of primary stem cell clone development was significantly higher (P<0.01) on buffalo and murine fibroblast monolayers as compared to the feeder free coated plates. The fibroblast monolayer was significantly better than feeder free coated plates in development of embryonic stem cell clones. It can be concluded that feeder cell layer culture system was found to be significantly better than feeder free culture system and matrigel coating plates were found to be better than fibronectin coating plate in development of embryonic stem (ES) cell clones.


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