2-Title: Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions
Authors: Gopal Puri and Sadhan Bag
Source: Ruminant Science (2012)-1(2):109-112.
How to cite this manuscript: Puri Gopal and Bag Sadhan (2012).Development of embryonic stem cell clone from in vitro derived buffalo embryos in feeder and feeder free culture conditions. Ruminant Science 1(2):109-112.
The present experiment was carried out to study the effects of feeder or feeder free culture system on developmental potency of in vitro derived buffalo embryos to embryonic stem cell clones. In vitro produced buffalo embryos were made zona free and cultured on two types of feeder layer (buffalo and murine fetal fibroblast monolayer) and two types of feeder free coated plates (matrigel and fibronectin extra cellular matrix) culture system. The percentage of primary stem cell clone development was significantly higher (P<0.01) on buffalo and murine fibroblast monolayers as compared to the feeder free coated plates. The fibroblast monolayer was significantly better than feeder free coated plates in development of embryonic stem cell clones. It can be concluded that feeder cell layer culture system was found to be significantly better than feeder free culture system and matrigel coating plates were found to be better than fibronectin coating plate in development of embryonic stem (ES) cell clones.
Dahéron L, Sarah L, Opitz SL, Zaehres H, William M, Lensch , WM, Andrews , PW, Itskovitz-Eldor J and Daley GQ (2004). LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells. Stem Cells 22:770-778.
Doetschman T, Williams P and Maeda N (1989). Establishment of hamster blastocyst derived embryonic (ES) cells. Development Biology 127:224-227.
Evans MJ and Kaufman MH (1981). Establishment of culture of pluripotent cells from mouse embryos. Nature 292:154-156.
Loh XJ, Gong J, Sakuragi M, Kitajima T, Liu M, Li J, Ito Y (2009). Surface coating with a thermoresponsive copolymer for the culture and non-enzymatic recovery of mouse embryonic stem cells. Macromolecular Bioscience 2009 Jun 15. [Epub ahead of print]
Martin GR and Evans MJ (1975). Differentiation of clonal lines of teratocarcinoma cells: Formation of embryoid bodies in vitro. Proceedings of the National Academy of Sciences 72:1441-1445.
Nath , NC (2010). Comparative study of totipotent stem cells derived from in vivo and in vitro produced goat embryos. PhD thesis submitted to IVRI, Demmed University, Izatnagar, Bareilly-243 122 (UP), India
Puck TT, Marcus PI and Cieciura SJ (1956). Clonal growth of mammalian cells in vitro: Growth characteristics of colonies from single HeLa cells with and without a feeder layer. Journal of Experimental Medicine 103:273-283.
Stewart CL (1991). Prospects for the establishment of embryonic stem cells-a genetic manipulation of domestic animals. In: Animal Application of Research in Mammalian Development. Eds RA Pedersen, A Mclaren and NL First, cold spring harbor laboratory press, NY, pp 267-283.
Suemori H and Nakatsuj N (1987). Establishment of the embryo derived stem cell lines from mouse blastocysts: Efects of the feeder dell layer. Development, Growth and Differentiation 29:133-139.
Vackova I, Ungrova A and Lopes F (2007). Putative embryonic stem cell lines from pig embryos. Journal of Reproduction and Development 53(6):1137-1149.
Wiles MV (1993). Embryonic stem cell differentiation in vitro. Methods in Enzymology 225:900-911.
Xu C, Inocuma MS, Denham J, Golds KS, Kundu P, Golds, JD and Carperter MK (2001). Feeder-free growth of undifferentiated human embryonic stem cells. Nature Biotechnology 19:971-974.
Zhang X, Neganova I, Przyborski S, Yang C, Cooke M, Atkinson SP, Anyfantis G, Fenyk S, Keith WN, Hoare SF, Hughes O, Strachan T, Stojkovic M, Hinds PW, Armstrong L and Lako M (2009). A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A. Journal of Cell Biology 184:67-82.