5-Title: Caffeine as semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation

Authors: MK Shukla, AK Misra and HP Gupta

Source: Ruminant Science (2012)-1(2):121-124.

How to cite this manuscript: Shukla MK, Misra AK and Gupta HP (2012). Caffeine as semen additive to improve Murrah buffalo (Bubalus bubalis) semen cryopreservation. Ruminant Science 1(2):121-124.


Caffeine was supplemented at a concentration of 1.0, 3.0 or 5.0 mM to Egg yolk tris glycerol extender (EYTG) at the time of dilution of Murrah buffalo bull semen which was subsequently frozen. The post thaw progressive motility and live sperm per cent improved significantly (P<0.05) with addition of 1.0 (70.0 and  and m  and caffeine to extender before semen dilution and cryopreservation as compared to control (61.94 1.15 and 79.05 1.02%), when evaluated at 0 h post freeze. All the three concentrations of caffeine significantly improved the Hypoosmotic swelling (HOS) % and protected the sperm from becoming abnormal as observed at 0 and 48 h after freezing. Thus incorporation of caffeine (1.0 or 3.0 mM) in EYTG extender, prior to freezing, may be useful in improving the quality of cryopreserved bubaline semen.


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