9-Title: Extreme ambiences associated variations in erythrocytic glutathione reductase levels in Marwari goat
Authors: M Chaturvedi, N Kataria and AK Kataria
Source: Ruminant Science (2012)-1(2):137-140.
How to cite this manuscript: Chaturvedi M, Kataria N and Kataria AK (2012). Extreme ambiences associated variations in erythrocytic glutathione reductase levels in Marwari goat. Ruminant Science 1(2):137-140.
Present investigation was carried out to find out extreme ambience associated variations in erythrocytic glutathione reductase levels in Marwari goats. For this purpose blood erythrocytes were collected from 630 apparently healthy Marwari goats of either sex, ageing 6 months to 4.5 years to prepare stroma free haemolysates to determine glutathione reductase levels. The mean value during moderate ambience was 0.795±0.02 kUgHb-1. A significant (p<0.05) increase was observed in the mean values during hot and cold ambiences as compared to moderate mean value. The sex and age effects were significant (p<0.05) in all ambiences. The mean values were significantly (p<0.05) higher in male animals than the female ones. In each ambience the effect of age showed a significant (p<0.05) increase in the mean values which was the highest in the animals of 2.0-4.5 years of age. The magnitude of increase in the values was higher in hot than cold ambience which suggested higher degree of oxidative stress in hot than in cold ambience. It was concluded that extreme ambiences produced oxidative stress in Marwari goats of both the sexes and all age groups, which was reflected in the form of altered status of the erythrocytic glutathione reductase. The evaluation of the extent of oxidative stress in the form of values can be useful to define the role of oxidative stress in different ambient conditions and can be used for clinical diagnosis and in health management.
Agar NS, Gruca MA and Harley JD (1974). Studies on glucose-6-phosphate dehydrogenase, glutathione reductase and regeneration of reduced glutathione in the red blood cells of various mammalian species. Australian Journal of Experimental Biology and Medical Science 52:607-614.
Agar NS, Suzuki T, Roberts J and Evans JV (1983). Effect of anaemia on red cell metabolism in cattle. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 75(3):445-449.
Braven J, Ansari N, Figgitt DP, Fisher A, Luders C, Hickling P and Whittaker M (1989). A comparison of glutathione reductase and glutathione peroxidase activities in patients with rheumatoid arthritis and healhy adults. Rheumatology 28:212-215.
D’Aguino M, Gaetani S and Spadoni MA (1983). Effect of factors of favism on the protein and lipid components of rat erythrocyte membrane. Biochimica et Biophysica Acta 731:161-167.
Duncan DB (1955). Multiple range and multiple F tests. Biometrika 11:1-42.
Goldberg B and Stern A (1977). The role of the superoxide anion as a toxic species in the erythrocyte. Archives of Biochimica et Biophysica Acta 178:218-225.
Jain NC (1986). In: Schalm’s Veterinary Haematology. 4th Edn, Lea and Febiger, Philadelphia.
Kataria N, Kataria AK, Joshi A, Pandey N and Khan S (2012). Serum antioxidant status to assess oxidative stress in brucella infected buffaloes. Journal of Stress Physiology and Biochemistry 8:5-9.
Kataria N, Kataria AK, Maan R and Gahlot AK (2010a). Evaluation of oxidative stress in brucella infected cows. Journal of Stress Physiology and Biochemistry 6(2):19-31.
Kataria N, Kataria AK and Maan R (2010b). Evaluation of oxidative stress due to hot environmental condition in healthy Marwari goats from arid tract in India. Philippine Journal of Veterinary and Animal Science 36(2):175-184.
Kataria N, Kataria AK, Pandey N and Gupta P (2010c). Serum biomarkers of physiological defense against reactive oxygen species during environmental stress in Indian dromedaries. HVM Bioflux 2:55-60.
King J (1965). In: Practical clinical enzymology. D Van Nostrand Company Ltd, London. pp 70-75.
Kurata M, Suzuki M and Agar NS (1993). Antioxidant systems and erythrocyte life-span in mammals. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 106(3):477-487.
Pinto RE and Bartley W (1969). The effect of age and sex on glutathione reductase and glutathione peroxidase activities and on aerobic glutathione oxidation in rat liver homogenates. Biochemistry Journal 112:109-115.
Rose RC and Bode AM (1993). Biology of free radical scavengers: An evaluation of ascorbate. Faseb Journal 7:1135-1142.
Russell LE, Easter RA and Bechtel PJ (1985). Evaluation of the erythrocyte aspartate aminotransferase activity coefficient as an indicator of the Vitamin B-6 status of postpubertal Gilts. Journal of Nutrition 115:1117-1123.
Steel RGD and Torrie JH (1980). In: Principles and procedures of statistics. A biometrical approach. 2nd Edn, McGraw-Hill Kogakusha Ltd, Tokyo. pp 187-190.
Trotta RJ, Sullivan SG and Stern A (1981). Lipid peroxidation and haemoglobin degradation in red blood cells exposed to t-butyl hydroperoxide. Dependence of glucose metabolism and haemoglobin status. Biochimica et Biophysica Acta 679:230-237.
Walsh MD, Kennedy DG, Goodall EA and Kennedy S (1993). Enzymatic effects of vitamin E or selenium depletion antioxidant enzyme activity in the muscles of calves depleted of vitamin E or selenium or both. British Journal of Nutrition 70:621-630.