10-Title: Effect of age and season on acrosomal integrity of Barbari buck sperm

Authors: A Kumar, DK Swain, T Kumar, J Kumar, S Yadav and V Pandey

Source: Ruminant Science (2013)-2(2):163-166.

How to cite this manuscript: Kumar A, Swain DK, Kumar T, Kumar J, Yadav S and Pandey V (2013). Effect of age and season on acrosomal integrity of Barbari buck sperm. Ruminant Science 2(2):163-166.

Abstract

Present study was proposed to evaluate the effects of age and season on the acrosomal integrity in Barbari bucks. Experiment was conducted in 18 bucks which were divided into 3 groups based on their age (G1- 7 months to less than 2 years, G2- 2 years to less than 5 years and G3- 5 years to 7.5 years). The three different seasons in which the experiment was conducted were spring (S1), winter (S2) and summer (S3) season. Semen was collected biweekly and 72 ejaculates were collected from each buck during the study period. The salient findings of the study were- all the three age groups showed significant difference (P<0.01) on the intactness of the acrosome in both pre-freeze and post-freeze semen; G3 showed the lowest intact acrosome per cent. In all the three seasons the bucks showed significant difference (P<0.01) on the intactness of the acrosome in both pre-freeze and post freeze semen; S3 showed the lowest intact acrosome per cent. From the study, it was concluded that both age and season affects the quality of sperm before freezing and after freezing in terms of acrosomal integrity.

References

Barkawi AH, Elsayed Eitedal H, Ashour G and Shehata E (2006). Seasonal changes in semen characteristics, hormonal profiles and testicular activity in Zaraibi goats. Small Ruminant Research 66:209-213.

Batista M, Nino T Alamo D,  Castro N, Santana M, Gonzalez F, Cabrera F and Gracia A (2009). Successful artificial insemination using semen frozen and stored by an ultrafreezer in the Majorera goat breed. Theriogenology 71:1307-1315

Brito LFC, Silva AEDF, Rodrigues LH, Vieira FV, Deragon LAG and Kastelic JP (2002). Effect of age and genetic group on characteristics of the scrotum, testes and testicular vascular cones, and on sperm production and semen quality in AI bulls in Brazil. Theriogenology 58(6):175-1186.

Bucak MN, Sarıozkan S, Tuncer PB, Ulutas PA and Akcadag HI (2009). Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation. Small Ruminant Research 81:90-95.

Chauhan MS, Kapila R, Gandhi KK and Anand SR (1994). Acrosome damage and enzyme leakage of goat spermatozoa during dilution, cooling and freezing. Andrologia 26:21-26.

Chen Z, Toth T, Godfrey-Bailey L, Mercedat N, Schiff I and Hauser R (2003). Seasonal variation and age-related changes in human semen parameters.  Journal of Andrology 24:226-231.

Dorado J, Munoz-Serrano A and Hidalgo M (2010).  The effect of cryopreservation on goat semen characteristics related to sperm freezability. Animal Reproduction Science 121:115-123.

Esteves CS, Sharma RK, Thomas AJ Jr and Agarwal A (2007). Evaluation of acrosomal status and sperm viability in fresh and cryopreserved specimens by the use of fluorescent peanut agglutinin lectin in conjunction with hypo-osmotic swelling test. International Brazilian Journal of Urology 33:364-76.

Javed MT, Khan A and Kausar R (2000). Effect of age and season on some semen parameters of Nili- Ravi buffalo (Bubalus bubalis) bulls. Veterinary Achieves 70:83-94.

Karagiannidis A, Varsakeli S and Karatzas G (2000). Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascus goat bucks born and raised in Greece. Theriogenology 53(6):1285-1293.

Kidd SA, Eskenazi B and Wyrobeck AJ (2001). Effects of male age on semen quality and fertility. Fertility and Sterility 75:237-248.

Maina VA, Chaudhari SUR, Mshelia GD and Williams A (2006). Influence of season on semen characteristics of Sahel bucks in Borno state. Journal of Applied Sciences 6(2):353-356.

Mendoza C, Carreras A, Moos J and Tesarik J (1992). Distinction between true acrosome reaction and degenerative acrosome loss by a one-step staining method using Pisum sativum agglutinin. Journal Reproduction Fertility 95:755-763.

Perez B and Mateos E (1996). Effect of photoperiod on semen production and quality in bucks of Verata and Malagueña breeds. Small Ruminant Research 23(1):23-28.

Roca J, Martinez E, Sanchez- Valverde MA, Ruiz S and Vazquez JM (1992). Seasonal variations of semen quality in male goats: Study of sperm abnormalities. Theriogenology 38:115-126.

Srinivas M, Naidu VK and Chetty AV (2002). Seasonal variation in physical characteristics of native buck (Capra hircus) semen in Andhra Pradesh. Indian Journal of Animal Reproduction 23(2):184-186.

Talebi J, Souri M, Moghaddam A, Karimi I and Miramahmoodi M (2009). Characteristics and seasonal variation in the semen of Markhoz bucks in western Iran. Small Ruminant Research 85:18-20.

Watson PF (2006). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science 60-61:481-492.