12-Title: Effect of zinc supplementation on histology of epididymis in Assam local goats (Capra hircus)
Authors: J Devi, J Goswami, BC Sarma and Kamal Sarma
Source: Ruminant Science (2013)-2(2):171-173.
How to cite this manuscript: Devi J, Goswami J, Sarma BC and Sarma Kamal (2013). Effect of zinc supplementation on histology of epididymis in Assam local goats (Capra hircus). Ruminant Science 2(2):171-173.
A total of thirty six healthy Assam local male goats of three months of age weighing between 3 to 4 kg were used for the present study. The kids were equally divided into three groups according to the feeding regime as group I (Control group) in which animals received no zinc supplementation, while animals of groups II and III received inorganic (Zinc sulphate at the level of 120 mg/kid/day) and organic form (Zinc propionate at the level of 40 mg/kid/day) of zinc supplements, respectively. Following supplementation, tissue pieces from three parts of the epididymis viz. caput, corpus and cauda, were fixed separately in 10 per cent neutral buffered formalin. All the tissues were processed for routine paraffin embedding tissue processing technique and stained with haematoxylin and eosin. Histological study of epididymis revealed no sperm pack in the epididymis till 4 months of age in all the groups. At 5 months of age, sperm pack was seen in caput, corpus and cauda of zinc-supplemented kids. At 6 and 7 months, the kids of all the groups had sperm pack in all the parts of the epididymis. Sperm pack was very compact in the cauda of organic group at 6 and 7 months of age. It was found that that early attainment of puberty in male kids could be achieved by supplementation of zinc. Organic zinc source proved better choice than inorganic one as feed supplement considering bioavailability and effectiveness.
Anderson MB, Lepak K, Farinas V and George WJ (1993). Protective action of zinc against cobalt induced testicular damage in the mouse. Reproductive Toxicology pp 49-54.
Bedwal RS and Bahuguna A (1994). Zinc, copper and selenium in reproduction. Cellular and Molecular Life Sciences 50:626-640.
Hambidge M (2003). Biomarkers of trace mineral intake and status. Journal of Nutrition 948S-955S.
Kuludin I (1983). Animal Breeding Abstracts 52:4697.
Kumar N, Verma RP, Singh LP, Varshney VP and Dass RS (2006). Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus ´ Bos taurus) bulls. Reproduction Nutrition Development 46: 663-675.
Luna LG (1968). Manual of Histological Staining Methods of Armed Forces Institute of Pathology. 3rd Edn, McGraw Hill Book Co, New York. pp 153-173.
McDowell LR, Conrad JH and Hembry R (1993). Minerals for Grazing Ruminants in Tropical Regions. 2nd Edn, Animal Science Department, University of Florida.
Miller WJ, Clifton CM and Fowler PR (1965). Influence of high levels of dietary zinc on zinc on milk, performance and biochemistry of lactating cows. Journal of Dairy Science 48:450-456.
Naidu KV and Pattabiraman SR (2002). Postnatal histological changes in epididymis of Madras Red ram lambs maintained under grazing and fed lot systems. Indian Journal of Animal Science 71:658-662.
Nilnophakoon N (1978). Histological studies on the regional post natal differentiation of the epididymis in the ram. Anatomia Histologia Embryologia 7:253-272.
Rojas LX, McDowell LR, Martin FG, Wilkinson NS, Johnson AB and Njeru CA (1996). Relative bioavailability of zinc methionine and two inorganic zinc sources fed to cattle. Journal of Trace Elements Medical Biology 10:205-209.
Sarlos PR, Molnar L, Ratky A and Jozsef (2005). Role of zinc supplement in weight gain, testicle development and semen characteristics of young rams. REU Technical Series 50: Sheep and Goat Production in Central and Eastern European Countries. pp 1-4.
Schanbacher BD, Gomes WR and Van Demark NL (1974).Developmental changes in spermatogenesis, testicular carnitine acetyl-transferase activity and sperm testosterone in the ram. Journal of Animal Science 39:889-892. (Animal Breeding Abstracts 43:1784).
Skinner JD (1974). Reproductive physiology of indigenous and exotic male animals in South Africa. Puberty in South African goat breeds, including the Angora. Agricultural Research (Pretoria), South Africa. (Animal Breeding Abstracts 43: 4628).
Underwood EJ and Somers M (1969). Studies of zinc nutrition in sheep I. The relation of zinc to growth, testicular development, and spermatogenesis in young rams. Australian Journal of Agricultural Research 20:889-895.