4-Title: Molecular detection of bovine rotavirus from north Gujarat, India
Authors: AI Dadawala, BS Chandel, HC Chauhan and AG Bhagat
Source: Ruminant Science (2013)-2(2):131-137.
How to cite this manuscript: AI Dadawala, BS Chandel, HC Chauhan and AG Bhagat (2013). Molecular detection of bovine rotavirus from north Gujarat, India. Ruminant Science 2(2):131-137.
Seventy six faecal samples were collected from diarrhoeic bovine calves up to 4 weeks of age to find out the prevalence of rotavirus associated with neonatal calf diarrhoea. All 76 samples were processed for detection of rotavirus antigen by LAT and rotavirus genome by AGE and RT-PCR. All 76 faecal samples were processed for detection of rotavirus antigens by using latex agglutination test, while agar gel electrophoresis and RT-PCR was employed for the detection of rotavirus genome. Of these, 14 (18.42%), 6(7.89%) and 6(7.89%) were found positive in LAT, AGE and RT-PCR, respectively.
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