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5 - Anandpub

5-Title: Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa

Authors: Abhay Raorane, Shubhadha Chothe, ZB Dubal, SB Barbuddhe, M Karunakaran, Swapnil Doijad, Ajay Pathak, Krupali Poharkar and NP Singh

Source: Ruminant Science (2013)-2(2):139-144.

How to cite this manuscript: Raorane Abhay, Chothe Shubhadha, Dubal ZB, Barbuddhe SB, Karunakaran M, Doijad Swapnil, Pathak Ajay, Poharkar Krupali and Singh NP (2013). Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa. Ruminant Science 2(2):139-144.

Abstract

Isolation and identification of pathogens associated with mastitis cases is of at most importance when it comes to the prevention and treatment aspects. Area specific antibiogram for specific mastitis pathogen will help to find out appropriate type of antimicrobial which will ultimately reduced the resistance of different antimicrobials. In the present study, 158 milk samples from six different dairy farms of Goa were collected aseptically and processed for isolation and identification of bacterial pathogens. Sixty seven samples were positive by California Mastitis Test excluding 12 samples from clinical cases of mastitis. A total of 51 isolates were recovered from CMT positive and clinical mastitis samples. Staphylococcus aureus (28.35%), coagulase negative Staphylococci (29.85%), Streptococcus spp. (10.44%) and E. coli (7.46%) were isolated from these samples. Antibiotic sensitivity tests by disc diffusion assay of Staphylococcus aureus (n=19) showed 100% susceptibility towards Chloramphenicol and Vancomycin. Out of 19 strains, 12 strains were found to have resistance towards maximum numbers of antimicrobials. Coagulase negative Staphylococci (CNS) showed moreover similar pattern with 100% sensitivity to chloramphenicol. Similarly, in case of Sterptococcus spp., all the isolates were sensitive to vancomycin while, 71.42% isolates were resistant to streptomycin and trimethoprim. E. coli isolates were completely resistance to ampicillin, vancomycin, carbanicillin and streptomycin. However, these strains were susceptible to trimethoprim and ciprofloxacin. Resistance towards antibiotics was observed in the order as, Carbanicilin>Ampicillin/Cloxacillin> Cephalexin >Ciprofloxacin.

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