5-Title: Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa
Authors: Abhay Raorane, Shubhadha Chothe, ZB Dubal, SB Barbuddhe, M Karunakaran, Swapnil Doijad, Ajay Pathak, Krupali Poharkar and NP Singh
Source: Ruminant Science (2013)-2(2):139-144.
How to cite this manuscript: Raorane Abhay, Chothe Shubhadha, Dubal ZB, Barbuddhe SB, Karunakaran M, Doijad Swapnil, Pathak Ajay, Poharkar Krupali and Singh NP (2013). Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa. Ruminant Science 2(2):139-144.
Abstract
Isolation and identification of pathogens associated with mastitis cases is of at most importance when it comes to the prevention and treatment aspects. Area specific antibiogram for specific mastitis pathogen will help to find out appropriate type of antimicrobial which will ultimately reduced the resistance of different antimicrobials. In the present study, 158 milk samples from six different dairy farms of Goa were collected aseptically and processed for isolation and identification of bacterial pathogens. Sixty seven samples were positive by California Mastitis Test excluding 12 samples from clinical cases of mastitis. A total of 51 isolates were recovered from CMT positive and clinical mastitis samples. Staphylococcus aureus (28.35%), coagulase negative Staphylococci (29.85%), Streptococcus spp. (10.44%) and E. coli (7.46%) were isolated from these samples. Antibiotic sensitivity tests by disc diffusion assay of Staphylococcus aureus (n=19) showed 100% susceptibility towards Chloramphenicol and Vancomycin. Out of 19 strains, 12 strains were found to have resistance towards maximum numbers of antimicrobials. Coagulase negative Staphylococci (CNS) showed moreover similar pattern with 100% sensitivity to chloramphenicol. Similarly, in case of Sterptococcus spp., all the isolates were sensitive to vancomycin while, 71.42% isolates were resistant to streptomycin and trimethoprim. E. coli isolates were completely resistance to ampicillin, vancomycin, carbanicillin and streptomycin. However, these strains were susceptible to trimethoprim and ciprofloxacin. Resistance towards antibiotics was observed in the order as, Carbanicilin>Ampicillin/Cloxacillin> Cephalexin >Ciprofloxacin.
References
Bauer AW, Kirby WMM, Sherris JC and Turck M (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45(4):493-496.
Dhanda MR and Sethi MS (1962). Investigation of mastitis in India. ICAR Research. Series No. 35, New Delhi, India.
Dua K (2001). Incidence, etiology and estimated economic losses due to mastitis in Punjab and in India-An update. Indian Dairyman 53(10):41-48
Dubal Z B, Rahman H, Pal P, Kumar A and Pradhan K (2010).characterization and antimicrobial sensitivity of the pathogens isolated from bovine mastitis with special reference to Escherichia coli and Staphylococcus spp. The Indian Journal of Animal Sciences 80(12):1163-1167.
Erskine RJ, Walker RD, Bolin CA, Bartlett PC and White DG (2002). Trends in antibacterial susceptibility of mastitis pathogens during a seven-year period. Journal of Dairy Science 85(5):1111-8.
Fontes CO, Silva VL, de Paiva MR, Garcia RA, Resende JA, Ferreira-Machado AB and Diniz CG (2013). Prevalence, antimicrobial resistance, and virulence characteristics of mecA-encoding coagulase-negative Staphylococci isolated from soft cheese in Brazil. Journal of Food Science 78(4):M594-9.
Frey Y, Rodriguez JP, Thomann A, Schwendener S and Perreten V (2013). Genetic characterization of antimicrobial resistance in coagulase-negative staphylococci from bovine mastitis milk. Journal of Dairy Science 96(4): 2247-57.
Fridkin S (2001). Vancomycin-intermediate and -resistant Staphylococcus aureus: what the infectious disease specialist needs to know. Clinical Infectious Disease 32(1):108-115.
Gharsa H, Ben Slama K, Gómez-Sanz E, Lozano C, Klibi N, Jouini A, Messadi L, Boudabous A and Torres C (2013). Antimicrobial resistance, virulence genes, and genetic lineages of Staphylococcus pseudintermedius in healthy dogs in Tunisia. Microbial Ecology DOI: 10.1007/s00248-013-0243-y
Guérin-Faublée V, Tardy F, Bouveron C and Carret G (2002).Antimicrobial susceptibility of Streptococcus species isolated from clinical mastitis in dairy cows. International Journal of Antimicrobial Agents 19(3):219-26.
Hillerton JE and Berry EA (2005). Treating mastitis in the cow-A tradition or an archaism. Journal of Applied Microbiology 98:1250-1255.
Hoşbul T, Bozdoğan B, Haznedaroğlu T and Ozyurt M (2013). Heterogeneous macrolide resistance in methicillin-resistant Staphylococcus aureus: investigation of resistance mechanisms and clonality. Mikrobiyoloji Bulteni 47(2):211-22.
Joshi S and Gokhale S (2006). Status of mastitis as an emerging disease in improved and periurban dairy farms. Annals of the New York Academy of Sciences 1081(1):74-83.
Kumar R, Yadav BR and Singh RS (2009). Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Current Microbiology 60(5):379-386.
Kumar R, Yadav BR and Singh RS (2010). Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Current Microbiology 60(5):379-86.
Makovec JA and Ruegg PL (2003). Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994-2001). Journal of the American Veterinary Medical Association 222(11):1582-1589.
Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS and Koo HC (2007). Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. Journal of Dairy Science 90(3): 1176-85.
Oliver SP and Murinda SE (2012). Antimicrobial resistance of mastitis pathogens. Veterinary Clinics of North America: Food Animal Practices 28(2):165-85.
Östensson K, Lam V, Sjögren N and Wredle E (2013). Prevalence of subclinical mastitis and isolated udder pathogens in dairy cows in Southern Vietnam. Tropical Animal Health and Production 45(4):979-86.
Petersson-Wolfe C, Mullarky I and Jones G (2010). Staphylococcus aureus mastitis: Cause, detection and control. Virginia Cooperative Extension, Publication 404-229. (http://pubs.ext.vt.edu/404/404-229/404-229.html)
Rahman A, Hossain MA, Paul SK, Sultana S, Haque N, Kabir MR and Hoque SM (2013). Methicillin-Resistant Coagulase-Negative Staphylococci (MRCoNS) by Disk Diffusion Method. Mymensingh Medical Journal 22(2):229-31.
Ranjan R, Gupta MK and Singh KK (2011). Study of bovine mastitis in different climatic conditions in Jharkhand, India. Veterinary World 4(5):205-208.
Sawant AA, Sordillo LM, Jayarao BM (2005). A survey on antibiotic usage in dairy herds in Pennsylvania. Journal of Dairy Science 88(8): 2991-2999.
Schalm OW and Noorlander DO (1957). Experiments and observations leading to development of the California mastitis test. Journal of the American Veterinary Medical Association 130:199–204
Sharma N, Maiti SK, and Koley KM (2004). Studies on the incidence of sub clinical mastitis in buffaloes of Rajnandgaon district of Chhattisgarh state. Veterinary Practitioner 5(2):123-124
Sharma NA, Gautam SR, Upadhyay K, Hussain JS, Soodan and Gupta SK (2006). Role of antioxidants in udder health: A review. Indian Journal of Field Veterinary 2(1):73-76.
Sharma N, Rho G, Hong Y, Kang T, Lee H, Hur T, and Jeong D (2012). Bovine Mastitis: An Asian Perspective. Asian Journal of Animal and Veterinary Advances 7(6):454-476.
Srinivasan V, Gillespie B, Lewis M, Nguyen L, Headrick I, Schukken Y and Oliver S (2007). Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli isolated from dairy cows with mastitis. Veterinary Microbiology 124(3-4):319-28.
Tenhagen BA, Hansen I, Reinecke A and Heuwieser W (2009). Prevalence of pathogens in milk samples of dairy cows with clinical mastitis and in heifersat first parturition. Journal of Dairy Research 76(2):179-87.
Teuber M (2001). Veterinary use and antibiotic resistance. Current Opinion in Microbiology 4(5):493-499.
Waters AE, Contente-CuomoT, Buchhagen J, Liu CM, Watson L, Pearce K, Foster JT, Bowers J, Driebe EM, Engelthaler DM, Keim PS and Price LB (2011). Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry. Clinical Infectious Diseases 52(10):1227-30.
Wei LH, Zou FM, Liu G, Deng JJ, Xue XD, Zhou JL, Jiang LY, Si XQ and Liu JL (2013). Drug resistance of methicillin resistant Staphylococcus strains in burn ward and relative analysis. Zhonghua Shao Shang Za Zhi 29(10):55-8.
Zadoks R and Fitzpatrick J (2009). Changing trends in mastitis. Irish Veterinary Journal 62(4):59-70.