9-Title: Vimentin identifies myoepithelial cells of buffalo mammary tissue
Authors: Ratan K Choudhary, Devendra Pathak, Dipak Deka and Ramneek
Source: Ruminant Science (2013)-2(2):157-161.
How to cite this manuscript: Choudhary Ratan K, Pathak Devendra, Deka Dipak and Ramneek (2013). Vimentin identifies myoepithelial cells of buffalo mammary tissue. Ruminant Science 2(2):157-161.
The present study examines if vimentin, an intermediate type filament, identify myoepithelial cells of buffalo mammary tissue. Myoepithelium, the basal cells of mammary epithelium, are oxytocin dependent that contracts alveoli during time of milk ejection. This study was carried out in nine buffalo mammary samples randomly collected from local slaughterhouse. Result of the study suggests that vimentin, a myoepithelial cell marker of murine, canine, bovine and human mammary tissues, also identifies myoepithelial cells of buffalo mammary gland. Frequency of myoepithelial cells (accessed by vimentin expression and expressed as proportion of myoepithelial cells of total epithelial cells counted) during dry period was lower (P=0.004) than the lactating period (5.55±1.1 vs. 23.38±2.89). Vimentin expression in mammary epithelial cells was restricted to basal or myoepithelial cells and none of the luminal or alveolar cells were immunopositive. Interestingly, prominent expression of vimentin was observed in myoepithelial cells and lymphocytes, macrophages and endothelial cells of blood vessels in infected gland of lactating animals. This study will help in identification and characterization of sub-type of mammary epithelium in order to understand the role of myoepithelial cells in buffalo mammary gland. Association of increased expression of vimentin and mastitis awaits further investigation.
Bhatia Y (2013). P-cadherin as myoepithelial cell marker for differential diagnosis of benign and malignant breast lesions. Indian Journal of Pathology and Microbiology 56:6-9.
Capuco AV, Akers RM and Smith JJ (1997). Mammary growth in Holstein cows during the dry period: Quantification of nucleic acids and histology. Journal of Dairy Science 80:477-87.
Coleman S, Silberstein SB and Daniel CW (1988). Ductal morphogenesis in the mouse mammary gland: Evidence supporting a role for epidermal growth factor. Developmental Biology 127:304-15.
Deugnier M, Teulière J, Faraldo MM, Thiery JP and Glukhova MA (2002). The importance of being a myoepithelial cell. Breast Cancer Research 4:224-230.
Faraldo MM, Teulière J, Deugnier MA, Taddei-De La Hosseraye I, Thiery JP and Glukhova MA (2005). Myoepithelial cells in the control of mammary development and tumorigenesis: data from genetically modified mice. Journal of Mammary Gland Biology and Neoplasia 10:211-219.
Fuchs E and Weber K (1994). Intermediate filaments: structure, dynamics, function, and disease. Annual Review of Biochemistry 63:345-82.
Glukhova M, Koteliansky V, Sastre X and Thiery JP (1995). Adhesion systems in normal breast and in invasive breast carcinoma. American Journal of Pathology 146:706-16.
Gudjonsson T, Adriance MC, Sternlicht MD, Petersen OW and Bissell MJ (2005). Myoepithelial cells: Their origin and function in breast morphogenesis and neoplasia. Journal of Mammary Gland Biology and Neoplasia 10:261-72.
Gudjonsson T, Rønnov-Jessen L, Villadsen R, Rank F, Bissell MJ and Petersen OW (2002). Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelialcells for polarity and basement membrane deposition. Journal of Cell Science 115:39-50.
Ivaska J, Pallari HM, Nevo J and Eriksson JE (2007). Novel functions of vimentin in cell adhesion, migration, and signaling. Experimental Cell Research 313:2050-62.
Mor-Vaknin N, Punturieri N, Sitwala K, and Markovitz DM (2003). Vimentin is secreted by activated macrophages. Nature Cell Biology 5:59-63.
Nagle RB, Böcker W, Davis JR, Heid HW, Kaufmann H, Lucas DO and Jarasch ED (1986). Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. Journal of Histochemistry and Cytochemistry34:869-81.
Otterbach F, Bànkfalvi A, Bergner S, Decker T, Krech R and Boecker W (2000). Cytokeratin 5/6 immunohistochemistry assists the differential diagnosis of atypical proliferations of the breast. Histopathology 37:232-40.
Rejman JJ, Hurley WL and Bahr JM (1989). Enzyme-linked immunosorbent assays of bovine lactoferrin and a 39-kilodalton protein found in mammary secretions during involution. Journal of Dairy Science 555-560.
Sternlicht MD, Kedeshian P, Shao ZM, Safarians, S and Barsky SH (1997). The human myoepithelial cell is a natural tumor suppressor. Clinical Cancer Research 3:1949-58.