9-Title: Vimentin identifies myoepithelial cells of buffalo mammary tissue

Authors: Ratan K Choudhary, Devendra Pathak, Dipak Deka and Ramneek

Source: Ruminant Science (2013)-2(2):157-161.

How to cite this manuscript: Choudhary Ratan K, Pathak Devendra, Deka Dipak and Ramneek (2013). Vimentin identifies myoepithelial cells of buffalo mammary tissue. Ruminant Science 2(2):157-161.

Abstract

The present study examines if vimentin, an intermediate type filament, identify myoepithelial cells of buffalo mammary tissue. Myoepithelium, the basal cells of mammary epithelium, are oxytocin dependent that contracts alveoli during time of milk ejection. This study was carried out in nine buffalo mammary samples randomly collected from local slaughterhouse. Result of the study suggests that vimentin, a myoepithelial cell marker of murine, canine, bovine and human mammary tissues, also identifies myoepithelial cells of buffalo mammary gland. Frequency of myoepithelial cells (accessed by vimentin expression and expressed as proportion of myoepithelial cells of total epithelial cells counted) during dry period was lower (P=0.004) than the lactating period (5.55±1.1 vs. 23.38±2.89). Vimentin expression in mammary epithelial cells was restricted to basal or myoepithelial cells and none of the luminal or alveolar cells were immunopositive. Interestingly, prominent expression of vimentin was observed in myoepithelial cells and lymphocytes, macrophages and endothelial cells of blood vessels in infected gland of lactating animals. This study will help in identification and characterization of sub-type of mammary epithelium in order to understand the role of myoepithelial cells in buffalo mammary gland. Association of increased expression of vimentin and mastitis awaits further investigation.

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