10-Title: Safety assessment of repeated intravenous ketoprofen administration based on hemato-biochemical alterations in sheep

Authors: SR Gondaliya, SK Bhavsar, RD Singh, JH Patel, RD Varia and AM Thaker

Source: Ruminant Science (2014)-3(2):181-184

How to cite this manuscript: Gondaliya SR, Bhavsar SK, Singh RD, Patel JH, Varia RD and Thaker AM (2014). Safety assessment of repeated intravenous ketoprofen administration based on hemato-biochemical alterations in sheep Ruminant Science 3(2):181-184.


Ketoprofen is a ‘non-selective cyclo-oxygenase inhibitor’ type of non-steroidal anti-inflammatory drug (NSAID) and used widely in the treatment of ruminants as anti-inflammatory, analgesic and antipyretic drug. The present investigation was undertaken to study impact of multiple doses of ketoprofen on hematological and biochemical parameters in sheep. Drug was administered intravenously at the dose rate of 3 mg/kg body weight in six Patanwadi sheep (weighing between 20 to 30 kg), repeated at 24 hours interval for 5 consecutive days. The mean values of hematological (hemoglobin, packed cell volume, total leukocyte count and differential leukocyte count) and biochemical parameters (serum alanine transaminase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total bilirubin, serum creatinine, blood urea nitrogen, blood glucose, serum albumin and total protein) in treated animals do not differ significantly (p>0.05) when compared to pre-treatment (control) samples. Values of serum aspartate transaminase measured at 3rd, 4th and 5th day were found significantly lower than the control (0 day) but were remained within normal range. Mean values of blood urea nitrogen at 1st, 2nd, 3rd, 4th and 5th day differed significantly (p<0.05) when compared to control values, but clinically no abnormalities were noticed. Thus, it was suggested that monitoring of kidney functions should be appraised during long term ketoprofen therapy in sheep.


Lees P, Giraudel J, Landoni MF and Toutain PL (2004). PK–PD integration and PK-PD modelling of nonsteroidal anti-inflammatory drugs: Principles and applications in veterinary pharmacology. Journal of Veterinary Pharmacology and Therapeutics 27:491-502.

MacAllister CG, Morgan SJ, Borne AT and Pollet RA (1993). Comparison of adverse effects of phenylbutazone, flunixin meglumine, and ketoprofen in horses. Journal of the American Veterinary Medical Association 202:71-77.

Narita T, Tomizawa N, Sato R, Goryoand M and Hara S (2005). Effects of long-term oral administration of ketoprofen in clinically healthy beagle dogs. Journal of Veterinary Medical Science 67(9):847-853.

Nazifi S, Rezakhani A and Maharloo FG (2002). Comparative study on the effects of flunixin meglumine and ketoprofen on haematological and some biochemical parameters of cattle. Journal of Veterinary Research (Tehran University) 57:95-99.

Paresh B, Singh R, Varia RD, Patel JH, Patel UD, Bhavsar SK and Thaker AM (2012). Impact of multiple intravenous administrations of ketoprofen on blood profile in cow calves. International Journal of Veterinary Science 1:34-36.

Riviere JE and Papich MG (2009). In: Veterinary Pharmacology and Therapeutics. 9th Edn, Wiley Blackwell, USA, pp: 457-492.

Singh RD, Sarita D, Gondaliya SR, Bhavsar SK and Thaker AM (2009). Safety of Ketoprofen in cow calves following repeated intravenous administration. Veterinary World 2:105-107.

Sweetman SC (2002). In: Martindale-The Complete Drug Reference. 33rd Edn, The Pharmaceutical Press, London, pp: 47-48.

Thompson L (2006). In: Merck Veterinary Manual. 51st Edn, Merck & Co, Inc, NJ, USA.

USP Monograph (2004). Monograph on Ketoprofen (Veterinary Systemic). The United States Pharmacopeial Convention.

Vane JR, Bakhle YS and Botting RM (1998). Cyclooxygenase 1 and 2. Annual Reviews in Pharmacology and Toxicology 38:97-120.