17-Title: Synthesis and conformational analysis of epsilon-poly-lysine
Authors: Arvind Kumar Singh, DR Harish, Shishir Kumar Gupta, Aditya P Sahoo, Satish Kumar and Ashok Kumar Tiwari
Source: Ruminant Science (2015)-4(2):199-203.
How to cite this manuscript: Singh Arvind Kumar, Harish DR, Gupta Shishir Kumar, Sahoo Aditya P, Kumar Satish and Tiwari Ashok Kumar (2015). Synthesis and conformational analysis of epsilon-poly-lysine. Ruminant Science 4(2):199-203.
In order to develop polypeptide based delivery vehicle to treat several diseases such as cancer in animals including ruminants, epsilon-poly-L-lysine (EPL), which is a cationic peptide, was synthesized by solution phase of peptide synthesis and characterized. EPL peptide was desalted by gel permeation chromatography (GPC) and characterized by mass spectroscopy and circular dichroism spectroscopy in polar and apolar environments. Although it is polydisperse, highest molecular mass of the peptide was 1800.285 dalton as observed in chromatogram. Conformational studies indicated that EPL acquired mainly beta sheet structure in aqueous as well as in apolar environment. The pKa value of alpha amine is 9.6 which get protonated at physiological pH which may help to bind to nucleic acid and cellular uptake.
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