21-Title: Biochemical profile of follicular fluid of Murrah buffaloes
Authors: MK Shukla, Dharmendra Kumar, Amita Tiwari, OP Shrivastava, SN Shukla and BC Sarkhel
Source: Ruminant Science (2015)-4(2):215-217.
How to cite this manuscript: Shukla MK, Kumar Dharmendra, Tiwari Amita, Shrivastava OP, Shukla SN and Sarkhel BC (2015). Biochemical profile of follicular fluid of Murrah buffaloes. Ruminant Science 4(2):215-217.
The pooled samples of follicular fluid was aspirated from small (<4 mm) and large (>4 mm) follicles of slaughter house ovaries of thirty Murrah buffaloes was centrifuged at 3000 rpm for 15 minutes and the supernatant was used for estimation of glucose, albumin, total protein and cholesterol. No significant difference was recorded in total protein and cholesterol of follicular fluid of small and large sized follicles. However, the glucose level significantly increased and albumin level decreased (p<0.01) with the increase of follicular size. It was concluded that biochemical contents of the follicular fluid were related with the growth of the follicles.
Abd Ellah MR, Hussein HA and Derar DR (2010). Ovarian follicular fluid constituents in relation to stage of estrus cycle and size of the follicle in buffalo. Veterinary World 3(6):263-267.
Andersen MMJ, Byskov AG and Faber M (1976). Protein composition in the fluid of individual bovine follicles. Journal of Reproduction and Fertility 48:109.
Bagavandoss P, Midgley AR and Wicha M (1983). Developmental changes in the ovarian follicular basal lamina detected by immunofluorescence and electron microscopy. Journal of Histochemistry and Cytochemistry 31:633-640.
Brantmeier SA, Grummer RR and Ax RL (1987). Concentrations of high-density lipoproteins vary among follicular sizes in the bovine. Journal of Dairy Science 70:2145-2149.
Caravaglios R and Cilotti R (1957). A study of the proteins in the follicular fluid of the cow. Journal of Endocrinology 15:273.
Gosden RG, Hunter RHF, Telfer E, Torrance C and Brown N (1988). Physiological factors underlying the formation of ovarian follicular fluid. Journal of Reproduction Fertility 82:7813-7825.
Hafez ESE and Hafez B (2008). Assisted reproductive technology. Reproduction in Farm Animals. 7th Edn, Part VI. Wiley Blackwell Publishing Company, US. p 418.
Khan FA, Das GK, Pande M, Mir RA and Shankar U (2011). Changes in biochemical composition of follicular fluid during reproductive acyclicity in a water buffalo (Bubalus bubalis). Animal Reproduction Science 127:38-42.
Leroy JL, Vanholder T, Delanghe JR, Opsomer G, Van Soom AB and de Kruif A (2004). Metabolite and ionic composition of follicular fluid from different sized follicles and their relationship to serum in dairy cows. Animal Reproduction Science 80:201-211.
McNatty KP (1978). Follicular fluid. In: The Vertebrate Ovary: Comparative Biology and Evolution. Eds: RE Jones. Plenum Press, New York, pp 215-259.
Meur SK, Yadav MC and Sanwal PC (1995). Biochemical analysis of buffalo follicular fluid in relation to follicular size and stage of estrous cycle. Journal of Veterinary Physiology and Allied Science 14(1):19-26.
Mishra OP, Pandey JN and Gawande PG (2008). Study on biochemical constituents of caprine ovarian follicular fluid after superovulation. Asian Australian Journal of Animal Science 16:1711-1715.
Rampure DN, Dalvi SH, Gudewar JD and Malpani BL (2014). Studies on thyroid hormones and some biochemical constituents of follicular fluid in buffalo. Journal of Buffalo Science 3(1):25-29.
Rufai N, Razzaque WAA and Shah A (2013). Biochemical parameters of follicular fluid in cyclic and acyclic sheep. Vetscan 7(2):15-20.
Schuetz AW and Aniswicz A (1974). Cation and protein composition of ovarian follicular fluid of the pig: Relation to follicular size. Biology of Reproduction 11:64-69.
Thangavel A and Nayeem M (2004). Studies on certain biochemical profile of the buffalo follicular fluid. Indian Veterinary Journal 81:25-27.
Wise T (1987). Biochemical analysis of bovine follicular fluid; albumin, total protein, lysosomal enzymes, ions, steroids and ascorbic acid content in relation to follicular size, rank, atresia classification and day of estrous cycle. Journal of Animal Science 64:1153-1169.