21-Title: Biochemical profile of follicular fluid of Murrah buffaloes

Authors: MK Shukla, Dharmendra Kumar, Amita Tiwari, OP Shrivastava, SN Shukla and BC Sarkhel

Source: Ruminant Science (2015)-4(2):215-217.

How to cite this manuscript: Shukla MK, Kumar Dharmendra, Tiwari Amita, Shrivastava OP, Shukla SN and Sarkhel BC (2015). Biochemical profile of follicular fluid of Murrah buffaloes. Ruminant Science 4(2):215-217.


The pooled samples of follicular fluid was aspirated from small (<4 mm) and large (>4 mm) follicles of slaughter house ovaries of thirty Murrah buffaloes was centrifuged at 3000 rpm for 15 minutes  and the supernatant was used for estimation of glucose, albumin, total protein and cholesterol. No significant difference was recorded in total protein and cholesterol of follicular fluid of small and large sized follicles. However, the glucose level significantly increased and albumin level decreased (p<0.01) with the increase of follicular size. It was concluded that biochemical contents of the follicular fluid were related with the growth of the follicles.


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