31-Title: B-mode intraocular echo-morphometry of Murrah buffalo (Bubalus bubalis)

Authors: S Singh, S Purohit and RP Pandey

Source: Ruminant Science (2015)-4(2):249-254.

How to cite this manuscript: Singh S, Purohit S and Pandey RP (2015). B-mode intraocular echo-morphometry of Murrah buffalo (Bubalus bubalis). Ruminant Science 4(2):249-254.


Transcorneal ultrasonographic scanning of live calf and adult healthy buffaloes (Bubalus bubalis) (n=6) were performed in an axial imaging plane to standardized the echomorphometric measurements of various ocular structures. Echo-morphological details of various intra-ocular structures were standardized. The echo-biometric studies on five parameters i.e. aqueous chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD), axial globe length (AGL) and scleroretinal rim thickness (SRRT) was conducted using 6-8 MHz convex transducer at 6-9 cm scanning depth with suitable gain. The average values of ACD, LT, VCD, AGL, SRRT was 1.98±0.05, 2.99±0.09, 8.71±0.08, 11.31±0.11, 13.34±0.14, 16.06±0.20, 26.45±0.30, 33.11±0.30, 2.30±0.04 and 2.68±0.05 mm, respectively in eyes of male and females of calf and adult buffalos. Non-significant (P<0.05) difference was observed in ACD, VCD, AGL and SRRT when compared left and right eye of male as well as female of calf and adult buffalo. LT was significantly (P<0.05) higher in female than male and VCD was non-significantly (P<0.05) lower in female than male. These standard echo-morphometric details provide a benchmark to diagnose the various ocular affections.


Assadnassab G and Fartashvand M (2011). Ultrasonography of vitreous chamber in Iranian Holstein cattle. Advances in Environmental Biology 5(7):1854-1856.

Assadnassab G and Fartashvand M (2013). Ultrasonographic evaluation of buffalo eyes. Turkish Journal of Veterinary and Animal Sciences, 37(4):395-398.

Boroffka SAEB, George V, Anne-Marie V and Erik T (2006). Intraobserver and interobserver repeatability of ocular biometric measurements obtained by means of B-mode ultrasonography in dogs. American Journal of Veterinary Research 67(10):1743-1749.

El-Maghraby H, Nyland TG and Bellhorn RW (1995). Ultrasonographic and biometric evaluation of sheep and cattle eyes. Veterinary Radiology and Ultrasound 36:148-151.

Gaiddon J, Rosolen SG, Steru L, Cook CS and Peiffer RJ (1991). Use of biometry and keratometry for determining optimal power for intraocular lens implants in dogs. American Journal of Veterinary Research 52(5):781-783.

Gilger BC, Davidson MG and Howard PB (1998). Keratometry, ultrasonic biometry, and prediction of intraocular lens power in the feline eye. American Journal of Veterinary Research 59(2):131-134.

Goddard PJ (1995). Veterinary Ultrasonography. CAB Interational. pp 1-12, 87-104, 165-275.

Gorig C, Varghese T, Stiles T, Broek J, Zagzebski JA and Murphy CJ (2006). Evaluation of acoustic wave propagation velocities in the ocular lens and vitreous tissues of pigs, dogs, and rabbits. American Journal of Veterinary Research 67(2):288-295.

Hillyer MH (1993). Ocular ultrasonography in the horse. In: The veterinary Annual. 33rd Edn. Eds: ME Raw and TJ Parkinson, Oxford, Blackwell Scientific Publications. pp 131-137.

Kealy JK and McAllister H (2000). Diagnostic Radiology and Ultrasonography of the Dog and Cat. 3rd Edn, WB Saunders Co., St. Louis. pp 1-20, 377-378.

Krohne SG, Gionfriddo J and Morrison EA (1998). Inhibition of pilocarpine-induced aqueous humor flare, hypotony, and miosis by topical administration of anti-inflammatory and anesthetic drugs to dogs. American Journal of Veterinary Research 59(4):482-488.

Mustafa TA (2005). The Bell’s phenomenon in new borns. Neurosciences 10(1):41-43.

Paunksnis A, Svaldeniene E, Paunksniene M and Babrauskiene V (2001). Ultrasonographic evaluation of the eye parameters in dogs of different age. Ultragarsas 2(39):1-4.

Potter TJ, Hallowell GD and Bowen IM (2008). Ultrasonographic anatomy of the bovine eye. Veterinary Radiology and Ultrasound 49(2):172-175.

Poulsen NC, Tobias R and Cartee RB (2000). An Atlas and Textbook of Diagnostic Ultrasongraphy of the Dog and Cat.1st Edn, Manson publishing, Hannover. pp 92-105.

Read RA and Barnett KC (1995). Equine glaucoma diagnosed with the aid of ultrasonography. Equine Veterinary Education 7(4):225-228.

Ribeiro AP, Santos NL, Silva VC, Campos AF, Teixeira A and Laus JL (2010). Ultrasonographic and ecobiometric findings in the eyes of adult goats. Ciência Rural 40(3):568-573.

Scotty NC, Cutler TJ, Brooks DE and Ferrell E (2004). Diagnostic ultrasonography of equine lens and posterior segment abnormalities. Veterinary Ophthalmology 7(2):127-139.

Shah HR, Reichel E and Busbee BG (2010). A novel lidocaine hydrochloride ophthalmic gel for topical ocular anesthesia. Local and Regional Anesthesia 3:57-63

Warrier S, Wu HM, Newland HS, Muecke J, Selva D, Aung T and Casson RJ (2008). Ocular biometry and determinations of refractive error in rural Myanmar: The Meiktila eye study. British Journal of Ophthalmology 92(12):1591-1594.

Whitcomb MB (2002). How to diagnose ocular abnormalities with ultrasound. American Association of Equine Practitioners 48:272-275.

Wilkie DA and Gilger BC (1998). Equine diagnostic ocular ultrasonography. In: Equine Diagnostic Ultrasonography. Eds: NW Rantanen and AO Mckinnon, 1st Edn, Baltimore, Williams and Wilkins, pp 637-643.

Williams JA  and Wilkie DA (1996). Ultrasonography of the eye. Comp. Cont. Educ. Pract. Vet., 18:667-676.

Wong TY, Foster PJ, Ng TP, Tielsch JM, Johnson GJ and Seah SK (2001). Variations in ocular biometry in adult Chinese population in Singapore: The Tanjong Pagar Survey. Invest. Ophthalmol. Vis. Sci., 42: 73-80.

Yadegari M, Salehi A, Ashtari A and Ashtari SM (2013). B-mode ultrasound biometry of intraocular structures in dromedary camels (Camelus dromedarius). Global Veterinaria 10(1):71-74