8-Title: Prevalence of Staphylococcus aureus associated sub-clinical mastitis in crossbred cows in Mizoram

Authors: Gunjan Das, C Lalnunpuia, K Sarma, SK Behera, TK Dutta and Samiran Bandyopadhyay

Source: Ruminant Science (2015)-4(2):167-170.

How to cite this manuscript: Das Gunjan, Lalnunpuia C, Sarma K, Behera SK, Dutta TK and Bandyopadhyay Samiran (2015). Prevalence of Staphylococcus aureus associated sub-clinical mastitis in crossbred cows in Mizoram. Ruminant Science 4(2):167-170.


Study was planned to determine the incidence, diagnosis and therapy of sub-clinical mastitis in crossbred cows (Holstein x Jersey). From the suspected samples characterization, isolation and antimicrobial sensitivity testing of the etiological agent particularly Staphylococcus aureus was done. Milk samples were collected from 75 quarters of 20 suspected lactating crossbred cows reared in private sheds. The incidence of sub-clinical mastitis was 90% (animal-wise) and 33.33% (quarter-wise) after employing CMT and WST respectively. A total of 50 S. aureus (66.67%) isolates were recovered from 75 suspected quarters after standard cultural examinations. The antibiogram of isolates revealed highest sensitivity to Enrofloxacin (64%) followed by Gentamicin (58%), Cloxacillin (56%), Pefloxacin (54%) respectively. Following the sensitivity trend of isolates, parenteral antibiotic therapy, enrofloxacin@5 mg/kg i.m and nonsteroidal antiinflammatory agent, [email protected] mg/kg s.c or i.v at 24-h interval for 5 days was given along with intra-mammary infusion of cloxacillin and colistin combination. There was therapeutic improvement in the affected quarters after one week of treatment.


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