Title: Effects of different oxygen concentrations on developmental potential of goat tetraploid embryos
Authors: JK Agrawal, A Saxena, SD Kharche, A Kumar, S Yadav and RP Pandey
Source: Ruminant Science (2016)-5(2):239-242.
Cite this reference as: Agrawal JK, Saxena A, Kharche SD, Kumar A, Yadav S and Pandey RP (2016). Effects of different oxygen concentrations on developmental potential of goat tetraploid embryos. Ruminant Science 5(2):239-242
The present study was designed to evaluate the effect of different O2 concentrations on the developmental potential of tetraploid embryos. For production of tetraploid embryo, 2-cell embryos were obtained by the method of in vitro fertilization. The produced 2-cell embryos were exposed to different O2 concentrations at 1.2 kV/cm field strength and 4 μs pulse duration and their fusion rate and subsequent developmental stages were compared. When the effect of different oxygen concentrations was evaluated, it was observed that maximum cleavage (82.35%) was recorded at the 5% oxygen concentration and 4.76% of the embryos developed upto the blastocyst stage. It was concluded that 5% oxygen concentration was the most suitable treatment for production of tetraploid embryos.
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