Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in murrah buffalo (Bubalus bubalis)

Title: Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in murrah buffalo (Bubalus bubalis)

Authors: Ashwani Arya, Archana Verma, ID Gupta, Dhaman Kumar, Ankit Magotra,

Mohsin Ayoub Mir and Arun Pratap Singh

Source: Ruminant Science (2016)-5(2):143-148.

Cite this reference as: Arya Ashwani, Verma Archana, Gupta ID, Kumar Dhaman, Magotra Ankit, Mir Mohsin Ayoub and Singh Arun Pratap (2016). Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in murrah buffalo (Bubalus bubalis). Ruminant Science 5(2):143-148.


Heat stress due to elevated temperature is a very important problem globally. The ultimate effect is on development, production and reproduction trait of animals. Breeding for heat stress tolerance can be mitigated by breeding animals having genotype that have improved levels of thermo-tolerance using different conventional and advanced genetic tools. Buffaloes exhibit signs of great heat stress when exposed to direct solar radiation and working in the sun during hot weather. Heat shock proteins (HSPs) expression has been correlated with resistance to stress and is considered as a potential indicator of animal adaptation to harsh environmental stress. HSP27 (HSPB1) gene is a candidate gene which plays an important role in thermotolerance, cytoprotection, chaperon activity and cell differentiation. The association with heat tolerance traits (respiration rate, rectal temperature and heat tolerance coefficient) were investigated in 100 Murrah buffaloes. One SNP in exon 1 at position 225 was confirmed in HSPB1 gene of Murrah buffalo. Effect of genotypes on RR, RT and HTC was not significant. Effect of THI and parity was significant for RT (P<0.0001). Heat tolerance coefficient (HTC) was also calculated to check the adaptability of the animals during the period of heat stress. THI had significant effect on RR, RT and HTC.


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