Effect of follicle size on glucose-6-phosphate dehydrogenase activity of buffalo oocytes

Title: Effect of follicle size on glucose-6-phosphate dehydrogenase activity of buffalo oocytes

Authors: MK Shukla, OP Shrivastava, D Kumar, SN Shukla, Neera Jain and BC Sarkhel

Source: Ruminant Science (2016)-5(2):243-245.

Cite this reference as: Shukla MK, Shrivastava OP, Kumar D, Shukla SN, Jain Neera and Sarkhel BC (2016). Effect of follicle size on glucose-6-phosphate dehydrogenase activity of buffalo oocytes. Ruminant Science 5(2):243-245.


Considering the insufficiency of morphological assessment for evaluation of oocytes, other evaluation parameters like glucose-6-phosphate dehydrogenase (G6PDH) activity are becoming popular. The present experiment was conducted to study the effect of follicular size on G6PDH activity of buffalo oocytes. The overall average of BCB+ (inactive G6PDH) and BCB- (active G6PDH) COCs in the present study was recorded to be 51.68±1.25 and 48.32±1.25%, respectively. The BCB+ COCs in large follicle (56.42±0.47) was significantly (p<0.01) higher as compared to small follicles (45.82±0.56). The reverse was observed in BCB- COCs where significantly (p<0.01) higher proportion of BCB- COCs was recorded in small follicle (54.18±0.56) as compared to large follicles (43.58±0.47), thus indicating better developmental competence of oocytes derived from large follicles.


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