Antibiotic resistance pattern of Staphylococcus aureus isolated from milk of cattle with clinical mastitis

Title: Antibiotic resistance pattern of Staphylococcus aureus isolated from milk of cattle with clinical mastitis

Authors: Sunita, Diwakar and AK Kataria

Source: Ruminant Science (2017)-6(2):319-322.

Cite this reference as: Sunita, Diwakar and Kataria AK (2017). Antibiotic resistance pattern of Staphylococcus aureus isolated from milk of cattle with clinical mastitis. Ruminant Science 6(2):319-322.


Staphylococcus aureus is the most significant causative agent of mastitis in dairy animals. In the present investigation, genotypically confirmed S. aureus isolates (n=30) from clinical mastitis cases in cows were subjected to antibiogram studies using 15 different antibiotics. Among 15 antibiotics used, cloxacillin was found to be 100% effective against all the isolates followed by meropenem (90%), gentamicin (83.33%), chloramphenicol (70%), levofloxacin (66.66%), norfloxacin and cefalothin (63.33% each) and cefaclor (56.66%). Highest resistance (56.66%) was shown towards linezolid followed by ticarcillin (50%) and cefaclor (40%).   


Asfour HAE and Darwish SF (2011). Phenotypic and Genotypic detection of both mecA and blaZ genes mediated â lactam resistance in Staphylococcus strain isolated from Bovine mastitis. Global Veterinaria 6(1):39-50.

Ayedin I, Kav K and Celik HA (2009). Identification and   antimicrobial susceptibility of subclinical mastitis pathogens isolated from hair goats’ milk. Journal of Animal and Veterinary Advances 8(6):1086-1090.

Bauer AW, Kirby WM, Sherris JC and Turck M (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45(4):493-496.

Bhati T, Kataria AK, Nathawat P, Sharma SK, Mohammed N and Mathur M (2013). Antimicrobial susceptibility profiling of Staphylococcus aureus isolates from bovine subclinical mastitis. Veterinary Research 6(2):39-42.

Ceniti C, Britti D, Santoro AML, Musarella R, Ciambrone  L, Casalinuovo  F and Costanzo N (2017). Phenotypic antimicrobial resistance profile of isolates causing clinical mastitis in dairy animals. Italian Journal of Food Safety 6:6612.

Cowan ST and Steel KJ (1975). In: Cowan and Steel’s Mannual for the identification of medical bacteria. Cambridge University Press, Cambridge.

Das G, Lalnunpuia C, Sarma K, Behera SK, Dutta TK and Bandyopadhyay S (2015). Prevalence of Staphylococcus aureus associated sub-clinical mastitis in crossbred cows in Mizoram. Ruminant Science 4(2):167-170.

Gomes F and Henriques M (2016). Control of bovine mastitis: Old and recent therapeutic approaches. Current Microbiology 72:377-82.

Haveri M, Roslöf A, Rantala L and Pyörälä S (2007). Virulence genes of bovine Staphylococcus aureus from persistent and nonpersistent intramammary infections with different clinical characteristics. Journal of Applied Microbiology 103(4):993-1000.

Katheria D, Gangwar LS, Rashmi and Kumar A (2016). Prospects and constraint faced by small holder dairy farmers and animal health service provider in controlling mastitis. Ruminant Science 5(1):51-54.

Kumar V, Patel JS, Patel BR, Mevada VK and Raval AP (2012). Therapeutic efficacy of antimicrobial drugs in clinical mastitis of cross bred cattle. Ruminant Science 1(2):177-180.

Langer A, Ahuja A and Bihani DK (2014). Diagnosis of mycotic mastitis in cow. Ruminant Science 3(2):235-236.

Nathawat P, Bhati T, Sharma SK, Mohammed N and Kataria AK (2013). Prevalence of Staphylococcus aureus in lactating goats with clinical mastitis and their antibiogram studies. Animal Biology and Animal Husbandry International Journal of the Bioflux Society 5(1):32-37.

Parmar VL, Prasad A, Patel JS, Dodiya PG, Javia BB and Mathpati BS (2015). Therapeutic management of clinical mastitis caused by Pseudomonas spp with special reference to homeopathy medicine in Jafarabadi buffalo – A case study. Ruminant Science 4(2):245-246.

Quinn PJ, Carter ME, Markey BK and Carter GR (1994). Clinical Veterinary Microbiology. Wolfe Publishing, Mosby-Year Book Europe Ltd. Lynton House, 7-12. Tavistock Square, London WCH 9LB, England.

Raguvaran R, Mondal DB, Jithin MV, Kumar B and Sivakumar M (2016). Staphylococcus aureus mastitis in a nondescript goat. Ruminant Science 5(2):291-292.

Raorane Abhay, Chothe S, Dubal ZB, Barbuddhe SB, Karunakaran M, Doijad S, Pathak A, Poharkar K and Singh NP (2013). Antimicrobial resistance of the pathogens isolated from bovine mastitis in Goa. Ruminant Science 2(2):139-144.

Rathore O, Rathore G, Patel C, Kinhekar AS, Mangwani N, Kumar V, Ravikumar RK and Kumar V (2016). Clinical evaluation of an indigenous practice against subclinical mastitis. Ruminant Science 5(1):95-98.

Kumar S and Kataria AK (2006). Antibiogram of Staphylococcus aureus isolates of cattle clinical mastitis origin. Veterinary Practitioner 7(2):123-125.

Sargeant JM, Scott HM, Leslie KE, Ireland MJ and Bashiri A (1998). Clinical mastitis in dairy cattle in Ontario: Frequency of occurrence and bacteriological isolates. Canadian Veterinary Journal 39:33-38.

Sharma SK, Nathawat P, Bhati T, Mohammed N, Chaudhary S, Raj  R, Solanki S and Kataria AK (2013). Characterization of Staphylococcus aureus isolated from nasal discharge from pneumonic camels (Camelus dromedarius). Animal Biology and Animal Husbandry International Journal of the Bioflux Society 5(1):38-43.

Sharma P, Reddy DP, Kumar PA, Gadicherla R, George N and Bhandari V (2015). Draft genome sequence of a Staphylococcus aureus strain isolated from a cow with clinical mastitis. Genome Announc 3(4):e00914-15.

Sindhu N, Sharma A and Jain VK (2008). Phenotypic and genotypic characterization of Staphylococcus aureus isolated from mastitis cases of cows and buffaloes. Journal of Immunology and Immunopathology 10(2):119-123.

Straub JA, Hertel C and Hammes WP (1999). A 23S rRNA target polymerase chain reaction based system for detection of Staphylococcus aureus in meat starter cultures and dairy products. Journal of Food Protection 62(10):1150-1156. 

Sudhan-thiramani S, Swetha CS and Bharathy S (2015). Prevalence of antibiotic-resistant Staphylococcus aureus from raw milk samples collected from the local vendors in the region of Tirupathi, India. Veterinary World 8(4):478-481.

Suma P, Swetha CS, Sudhanthiramani, Goud S, Supriya A and Babu AJ (2016). A study on the antibiotic resistance patterns of Staphylococcus aureus isolated from market milk in and around Tripiti, Andhra-pradesh. International Journal of Recent Scientific Research 7(4):10429-10435.

Wang Y, Wu CM, Isolated Lu LM, Ren GWN, Cao XY and Shen JZ (2008). Macrolide-lincosamide resistant phenotypes and genotypes of Staphylococcus aureus isolated from bovine clinical mastitis. Veterinary Microbiology 130(1-2)118-125.

Watts  JL (1988). Etiological agents of bovine mastitis. Veterinary Microbiology 16:41-66.

Yadav R, Sharma SK, Yadav J, Choudhary S and Kataria AK (2015). Profiling of antibiotic resistance of Staphylococcus aureus obtained from mastitic milk of cattle and buffalo. Journal of Pure and Applied Microbiology 9(2):1539-1544.