Effect of thermal stress on blood plasma levels of caspases and HSPS in Tharparkar and Karan Fries cattle

Title: Effect of thermal stress on blood plasma levels of caspases and HSPS in Tharparkar and Karan Fries cattle

Authors: Uttarani Maibam, OK Hooda, PS Sharma and RC Upadhyay

Source: Ruminant Science (2017)-6(2):243-246.

Cite this reference as: Maibam Uttarani, Hooda OK, Sharma PS and Upadhyay RC (2017). Effect of thermal stress on blood plasma levels of caspases and HSPS in Tharparkar and Karan Fries cattle. Ruminant Science 6(2):243-246.


The study aimed to investigate the effect of thermal stress on apoptosis related proteins and heat stress markers in Tharparkar and Karan fries cattle. Ten each of Tharparkar (Bos indicus) and Karan Fries (Bos indicus x Bos taurus) heifers, maintained under standard managemental practices, were selected from NDRI herd, Karnal. Blood samples were aseptically collected from each animal during winter, spring and summer. Apoptosis related enzymes (caspase 3 and caspase 7) and heat stress markers (HSP70.1 and HSP70.2) were determined by ELISA. In both the breeds, higher (P<0.05) levels of caspase 3, caspase 7 and HSP70s were observed during summer followed by winter and spring. Breeds showed no significant difference during winter and spring. During summer, caspase 3, caspase 7 and HSP70s were higher (P<0.05) in Karan Fries than Tharparkar. The study concludes that plasma level of caspases, and thus apoptosis was strongly affected by thermal stress. Caspases levels were higher in Karan Fries with higher levels of HSPs than Tharparkar during summer. This might be a due to difference in heat tolerance of Karan Fries (crossbred) and Tharparkar (zebu).


Adachi S, Kokura S, Okayama T, Ishikawa T, Takagi T, Handa O, Naito Y and Yoshikawa T (2009). Effect of hyperthermia combined with gemcitabine on apoptotic cell death in cultured human pancreatic cancer cell lines. International Journal of Hyperthermia 25:210-219.

Altan O, Pabuccuoglu A, Altan A, Konyalioglu S and Bayraktar H (2003). Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. British Poultry Science (44):545-550.

Arya Ashwani, Verma Archana, Gupta ID, Kumar Dhaman, Magotra Ankit, Mir MAand Singh AP (2016). Influence of parity, THI and HSPB1 gene SNP on heat tolerance indicator traits in murrah buffalo (Bubalus bubalis). Ruminant Science 5(2):143-148.

Bijur GN, DeSarno P and Jope RS (2000). Glycogen synthase kinase-3b facilitates staurosporine- and heat shock-induced apoptosis. Protection by lithium. Journal of Biological Chemistry 275:7583-7590.

Collier RJ, Collier JL, Rhoads RP and Baumgard LH (2008). Genes involved in the bovine heat stress response. Journal of Dairy Science 91:445-454.

De Maio A (1999). Heat shock proteins: facts, thoughts, and dreams. Shock (Augusta Ga) 11(1):1-12.

Jin Y, Li JY, Choi SH, Kim T, Cui X and Kim N (2007). Heat shock inducing apoptosis related gene expression and apoptosis in porcine parthenotes developing in vitro. Animal Reproduction Science 100:118-127.

Johnson HD, Ragsdale AC, Berry, IL, Shanklin MD (1963). Temperature-humidity effects including influence of acclimation in feed and water consumption of Holstein cattle. Research Bulletin of Missouri Agricultural Experiment Station  No. 846, p 43.

Johnson TE, Lithgow GJ and Murakami S (1996). Hypothesis: Interventions that increase the response to stress offer the potential for effective life prolongation and increased health Journals of Gerontology Series A Biological Sciences and Medical Sciences 51(6):B392-395.

Krininger CE, Stephens SH and Hansen PJ (2002). Developmental changes in inhibitory effects of arsenic and heat shock on growth of pre-implantation bovine embryos. Molecular Reproduction and Development 63(3):335-340.

Kvitt H, Rosenfeld H and Tchernov D (2016). The regulation of thermal stress induced apoptosis in corals reveals high similarities in gene expression and function to higher animals. Scientific Reports 6:30359.

Lacetera N, Bernabucci U, Scalia D, Basirico L, Morera P and Nardone A (2006). Heat stress elicits different responses in peripheral blood mononuclear cells from Brown Swiss and Holstein cows. Journal of Dairy Science 89:4606-4612.

Li ZH and Wang L (2009). Influence of heat stress on lymphocyte apoptosis of Holstein cow. Hebei Agricultural Science (48):35-42.

Ozben T (2007). Oxidative stress and apoptosis: impact on cancer therapy. Journal of Pharmaceutical Sciences 96:2181-2196.

Singh AK, Upadhyay RC, Malakar D, Kumar S and Singh SV (2014). Effect of thermal stress on HSP70 expression in dermal fibroblast of zebu (Tharparkar) and crossbred (Karan-Fries) cattle. Thermal Biology 43:46-53.

Vijayan MM, Pereira C, Kruzynski G and Iwama GK (1998). Sublethal concentrations of contaminant induce the expression of hepatic hsp70 in two salmonids. Aquatic Toxicology 40:101-108.

Yadav A, Singh KP, Singh MK, Saini N, Palta P, Manik RS, Singla SK, Upadhyay RC and Chauhan MS (2013). Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro. Reproduction in Domestic Animals 48(5):858-65.

Yadav VP and Rai A (2016). Effect of environment on gene expression profiling of TNF alpha in barbari goats (Capra hircus). Ruminant Science 5(2):159.