Title: Effect of SSCP variants of lactoferrin gene, parity and stage of lactation on milk lactoferrin and somatic cell count in Murrah buffaloes

Authors: Arun Pratap Singh, KP Ramesha, MA Mir, Ashwani Arya and S Isloor

Source: Ruminant Science (2018)-7(2):171-178.

Cite this reference as: Singh AP, Ramesha KP, Mir MA, Arya Ashwani and Isloor S (2018). Effect of SSCP variants of lactoferrin gene, parity and stage of lactation on milk lactoferrin and somatic cell count in Murrah buffaloes. Ruminant Science 7(2):171-178.

Abstract

The present study aimed at polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in Murrah (Bubalus bubalis) buffaloes to identify single nucleotide polymorphisms (SNPs) lactoferrin gene and to analyse the association between the observed polymorphisms with milk lactoferrin content and Somatic cell count (SCC). PCR-SSCP analysis revealed a total of four different variants in the coding region of the lactoferrin gene. PCR-SSCP analysis of exon 13 and 16 in the lactoferrin gene revealed two unique patterns each at the same time remaining five contrasting exons exhibited the monomorphic pattern. SSCP patterns of exon 13 and 16 had a significant effect on milk SCC (P<0.05).  The SCC and lactoferrin content in the milk of Murrah buffaloes was highest in 4th and above parity group. Stage of lactation had highly significant (P<0.01) effect on both milk SCC and lactoferrin content. The correlation between SSC and lactoferrin content in milk was significant and high(r=0.741; p<0.01). The association between SSCP variants with milk SCC and milk lactoferrin content can be used as markers for selection of animals for low somatic cell count, as a marker of susceptibility/resistance to mastitis in Murrah buffaloes.

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