Blood plasma enzymatic profiles as predictor of post-obstetrical survival of torsion affected Mehsana buffaloes

7-Title: Blood plasma enzymatic profiles as predictor of post-obstetrical survival of torsion affected Mehsana buffaloes

Authors: PM Chauhan, VK Sharma, BN Suthar, HC Nakhashi and TV Sutaria

Source: Ruminant Science (2019)-8(2):181-184

How to cite this manuscript: Chauhan PM, Sharma VK, Suthar BN, Nakhashi HC and Sutaria TV (2019). Blood plasma enzymatic profiles as predictor of post-obstetrical survival of torsion affected Mehsana buffaloes. Ruminant Science 8(2):181-184.


The present study was conducted on 78 torsion affected Mehsana buffaloes divided into survived (n=52) and non-survived groups (n=26) following delivery of the fetus to evaluate the blood plasma levels of AST, ALT, ALP, ACP, LDH and CK enzymes activity as predictor of post-obstetrical survival of dam. The blood samples from buffaloes of both groups were collected initially on day of hospitalization, after delivery of the fetus followed by 24 hour later and then on day 3, 5 and 10 of obstetrical maneuvers to obtain plasma for estimation of enzyme levels. The normal parturient buffaloes (n=6) were also bled on similar intervals for comparison of data. The average values of these enzymes remained significantly elevated (p<0.05) in torsion affected survived and non-survived groups as compared to normal parturients. Besides, the non-survived buffaloes had further higher concentrations of AST, ACP and CK enzymes than those of survived on day of hospitalization (160.04±20.14, 136.14±4.58 and 697.59±181.22 vs 130.11±8.13, 112.13±2.44 and 387.75±38.79 U/L), after delivery of the fetus (189.02±22.40, 141.12±7.52 and 710.75±218.57 vs 124.94±10.52, 112.07±2.56 and 348.64±38.55 U/L) and 24 hour later to delivery (313.14±66.39, 133.49±8.59 and 979.62±352.32 vs 184.80±17.20, 116.48±2.87 and 489.46±46.74 U/L) while LDH levels were higher up to day 3 (2222.70±84.15 vs 1416.63±37.11 U/L). The values of CK enzyme within the group of survived buffaloes declined significantly on day 3 (300.79±38.23 U/L) and were low up to day 10 (188.65±25.80 U/L) whereas significantly higher level of ALP (214.15±7.36 U/L) after 24 hour of fetal delivery and AST on day 3 (313.14±66.39 U/L) were evident in non-survived buffaloes. It is concluded that lower levels of AST, ACP and CK enzymes with an average of 184.80±17.20, 116.48±2.87 and 489.43±46.74 U/L, respectively critically after 24 hour of the fetal delivery and LDH level of 1416.63±57.11 U/L on day 3 of obstetrical delivery in survived group might be used to predict the dam survival.


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