7-Title: Blood plasma enzymatic profiles as predictor of post-obstetrical survival of torsion affected Mehsana buffaloes
Authors: PM Chauhan, VK Sharma, BN Suthar, HC Nakhashi and TV Sutaria
Source: Ruminant Science (2019)-8(2):181-184
How to cite this manuscript: Chauhan PM, Sharma VK, Suthar BN, Nakhashi HC and Sutaria TV (2019). Blood plasma enzymatic profiles as predictor of post-obstetrical survival of torsion affected Mehsana buffaloes. Ruminant Science 8(2):181-184.
The present study was conducted on 78 torsion affected Mehsana buffaloes divided into survived (n=52) and non-survived groups (n=26) following delivery of the fetus to evaluate the blood plasma levels of AST, ALT, ALP, ACP, LDH and CK enzymes activity as predictor of post-obstetrical survival of dam. The blood samples from buffaloes of both groups were collected initially on day of hospitalization, after delivery of the fetus followed by 24 hour later and then on day 3, 5 and 10 of obstetrical maneuvers to obtain plasma for estimation of enzyme levels. The normal parturient buffaloes (n=6) were also bled on similar intervals for comparison of data. The average values of these enzymes remained significantly elevated (p<0.05) in torsion affected survived and non-survived groups as compared to normal parturients. Besides, the non-survived buffaloes had further higher concentrations of AST, ACP and CK enzymes than those of survived on day of hospitalization (160.04±20.14, 136.14±4.58 and 697.59±181.22 vs 130.11±8.13, 112.13±2.44 and 387.75±38.79 U/L), after delivery of the fetus (189.02±22.40, 141.12±7.52 and 710.75±218.57 vs 124.94±10.52, 112.07±2.56 and 348.64±38.55 U/L) and 24 hour later to delivery (313.14±66.39, 133.49±8.59 and 979.62±352.32 vs 184.80±17.20, 116.48±2.87 and 489.46±46.74 U/L) while LDH levels were higher up to day 3 (2222.70±84.15 vs 1416.63±37.11 U/L). The values of CK enzyme within the group of survived buffaloes declined significantly on day 3 (300.79±38.23 U/L) and were low up to day 10 (188.65±25.80 U/L) whereas significantly higher level of ALP (214.15±7.36 U/L) after 24 hour of fetal delivery and AST on day 3 (313.14±66.39 U/L) were evident in non-survived buffaloes. It is concluded that lower levels of AST, ACP and CK enzymes with an average of 184.80±17.20, 116.48±2.87 and 489.43±46.74 U/L, respectively critically after 24 hour of the fetal delivery and LDH level of 1416.63±57.11 U/L on day 3 of obstetrical delivery in survived group might be used to predict the dam survival.
Bugalia NS, Sharrna DK, Phogat JB, Kuhad KS and Bansal SR (1996). Variation in biochemical constituents in bovine dystocia due to uterine torsion and monstrosities. Indian Veterinary Journal 73:839-843.
Constable P, Hinchcliff KW, Done S, Gruenberg W (2016). Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 11th Edn, The English Language Book Society and Bailliere Tindall, London, U.K.
Dhindsa SS, Dhaliwal GS, Sood NK and Ghuman SPS (2009). Blood biochemical and uterine tissue alterations in relation to duration of dystocia in caesarean-operated bovines. Indian Journal of Animal Science 79:30-33.
Dutta JC and Dugwekar YG (1982). Serum alkaline phosphatase and lactic dehydrogenase activity in cows with retained fetal membrane. Theriogenology 18(4):423-429.
El-Din Zain A, EI-Ballal SS and Shehata SH (1997). Alkaline phosphatase and transaminase activity in uterine flushing of buffalo-cows affected with endometritis. Buffalo Journal 13(1):95-102.
Ghuman SPS (2010). Uterine torsion in bovines: A review. Indian Journal of Animal Science 80:289-05.
Highman B and Atland RD (1960). Serum enzymes rise after hypoxia and effect of autonomic blockade. American Journal of Physiology 199:981-986.
Hussein H and Abd Ellah MR (2008). Effects of dystocia, fetotomy and caesarian sections on the liver enzymes activities and concentrations of some serum biochemical parameters in dairy cattle. Animal Reproduction Science 105:384-391.
Kaur T and Singh B (1993). Reproductive disorders in buffaloes: Hemato-biochemical studies. Indian Journal of Veterinary Pathology 17(2):118-121.
Manju TS, Verma SK, Gupta RC, Mandakhot VM and Krishnaswamy A (1985). Profiles of some plasma biochemical constituents associated with uterine torsion and following its correction by laparohysterotomy in buffaloes. Indian Journal of Animal Reproduction 6:57-61.
Page HM, Erwin, ES and Nelms GE (1960). Effect of solar radiation on variations of blood consitituents in cattle. Journal of Dairy Science 43:99-100.
Patel SC, Patel MP, Desai SB and Nakhashi HC (2012). Haematic mummification associated with uterine torsion in a Kankrej cow. Ruminant Science 1(2):199-200.
Phogat JB, Bugalia NS, Verma SK and Singh N (1995). Plasma transaminases, phosphatases and dehydrogenase activities in buffaloes affected with uterine torsion. Indian Journal of Animal Sciences 65(1):56-58.
Prabhakar S, Nanda AS and Ghuman SPS (2002). Changes in plasma cortisol concentrations as an index of stress due to dystocia and obstetrical manoevering in buffaloes. Indian Journal of Animal Science 72:309-311.
Purohit GN, Mehta JS, Sharma Sunanda, Shekher Chandra, Kumar Pramod , Kumar Amit, Raiya Sunil, Ruhil Swati and Gaur Mitesh (2013). Uterine torsion in buffaloes: A retrospective analysis of 52 cases. Ruminant Science 2(2):219-222.
Reitman S and Frankel S (1957). Glutamic-pyruvate transaminase assay by colorimetric method. American Journal of Clinical Pathology 28:56.
Roberts SJ (1986). Veterinary Obstetrics and Genital Diseases. 2nd Edn, CBS Publishers and Distributors, New Delhi.
Snedecor GW and Cochran WG (1994). Statistical Methods. 8th Edn, Iowa State University Press, Ames, Iowa.
Saraswat Chandrashekher, Dholpuria Sandeep, Jhamb Dinesh, Gaur Mitesh and Sharma Sunandha (2015). Clinico-obstetrical approaches on uterine torsion cases in Surti buffalo of tribal area of southern Rajasthan. Ruminant Science 4(2):225-230.
Sutaria TV, Kapadiya PS, Nakhashi HC, Chauhan PM, Suthar BN and Sharma VK (2015). Study on treatment efficacy of uterine torsion in Mehsana buffaloes. Ruminant Science 4(1):109-111.
Tietz NW, Rinker AD and Shaw LM (1983). IFCC methods for the measurement of catalytic concentration of enzymes. Part 5. IFCC method for alkaline phosphatase. Journal of Clinical Chemistry and Clinical Biochemistry 21:731-748.
Tripathi SS (1970). Effects of protein deficiency on physiology and biochemistry of male reproductive organs. PhD Thesis submitted to Agra University, Agra.