Comparative therapeutic management of anoestrus using Ovsynch and insulin plus Ovsynch protocol

8-Title: Comparative therapeutic management of anoestrus using Ovsynch and insulin plus Ovsynch protocol in Mehsana buffaloes

Authors: AI Chaudhari, HC Nakhashi, TV Sutaria, RK Chaudhari, DN Patel, CF Chaudhari and BN Suthar

Source: Ruminant Science (2019)-8(2):185-188

How to cite this manuscript: Chaudhari AI, Nakhashi HC, Sutaria TV, Chaudhari RK, Patel DN, Chaudhari CF and Suthar BN (2019). Comparative therapeutic management of anoestrus using Ovsynch and insulin plus Ovsynch protocol in Mehsana buffaloes. Ruminant Science 8(2):185-188.



The estrus induction, intensity of estrus, fertility response as well as status of endocrine and plasma growth factor profile were studied in 20 postpartum anoestrus Mehsana buffaloes treated with Ovsynch protocol (Group-I, n=10) and Insulin plus Ovsynch protocol (Group-II, n=10). The per cent estrus induction and conception rate were 100 & 100 and 60 & 50 in Group-I and Group-II, respectively. The number of services per conception was lower in Insulin plus ovsynch (2.33) as compared to ovsynch protocol (2.40). The 66.66 per cent buffaloes conceived with first two services in Insulin plus ovsynch. Average days at fertile estrus post treatment were observed to be 47.00 and 42.16 in conceived buffaloes of Group-I and Group-II, respectively. There was significantly higher P4 concentration in conceived (3.70±0.15 and 3.53±0.07) than non-conceived (1.89±0.15 and 1.83±0.08) sub groups of both the treatment groups buffaloes on day 21st. The concentration of IGF-I was significantly (P<0.05) higher on day 9th as compared to day 7th in both the groups. Ovsynch and insulin plus Ovsynch protocols can be used to induce the estrus in post-partum anestrus buffaloes with a positive effect of insulin supplementation.


Agrawal Jitendra Kumar, Saxena Atul, Kharche SD, Pathak Juhi, Sikarwar AKS, Patel Akhil, Goel AK and Jindal SK (2016). A study on parthenogenetic activation and embryo production from in vitro matured caprine oocytes. Ruminant Science 5(1):21-24.

Bisen A, Shukla SN, Shukla MK and Mishra A (2018). Fertility response using timed insemination protocols in sub-oestrus buffaloes. Journal of Animal Research 8(3):417-421.

Dudi V, Mehta JS, Chaudhary AK, Kumar Pramod, Kumar Amit and Ruhil Swati (2017). Efficacy of different estrus synchronizing protocols on estrus induction in postpartum lactating dairy cows. Ruminant Science 6(2):337-340.

Ghuhan SPS, Honparkhe M and Singh J (2014). Comparison of ovsunch and progesterone-based protocol for induction of synchronized ovulation and conception rate in subestrous buffalo during low breeding season. Iranian Journal of Veterinary Research 49(15):375-378.

Gupta KK, Shukla SN, Inwati P and Shrivastava OP (2015). Fertility response in postpartum anoestrus buffaloes (Bubalus bubalis) using modified Ovsynch based timed insemination protocols. Veterinary World 8(3):316-319.

Jainudeen MR (2000). Buffalo Reproduction. In: Reproduction in Farm Animals. Eds: ESE Hafez. 8th Edn, Lea and Febiger, Philadelphia, p 193.

Kumar A, Sharma U, Pandey A, Kumar S and Kumar S (2015). Induction of ovarian cyclicity and conception rate in postpartum anestrus buffaloes treated with different hormonal protocols. Buffalo Bulletin 34(4):441-445.

Kumar Amit, Mehta JS, Ruhil Swati and Purohit GN (2018). Effects of different estrous synchronization protocols on estrus and subsequent fertility in cycling cows. Ruminant Science 7(1):83-86.

Minegishi T, Hirakawa T, Kishi H, Abe K, Abe Y, Mizutani T and Miyamoto K (2000). A role of insulin-like growth factor-I for follicle-stimulating hormone receptor expression in rat granulosa cells. Biology of Reproduction 62:325-333.

Nakrani BB, Panchal MT, Dhami AJ, Hadiya KK, Patel JA and Gosai RK (2014).  Effect of controlled breeding techniques on fertility and plasma profile of biochemical and mineral constituents in anoestrus buffaloes. Scholars Journal of Agriculture and Veterinary Sciences 1(4):299-304.

Palma GA, Muller M and Brem G (1997). Effect of insulin-like growth factor I (IGF-I) at high concentrations on blastocyst development of bovine embryos produced in vitro. Journal of  Reproduction and Fertility 110:347-353.

Palta P and Madan ML (1995). Alteration in hypophyseal responsiveness to synthetic GnRH at different postpartum interval in Murrah buffaloes (Bubalus bubalis). Theriogenology 44:803-811.

Parmar BN, Patel BM, Vijyeta HP and Parikh SS (2017). Controlled breeding techniques for enhancing reproductive performance of buffaloes. Indian Veterinary Journal 94(5):33-35.

Patelia HR (2018). Efficiency of modified ovsynch protocol in post-partum Mehsana buffaloes with special reference to the use of double dose of GnRH. MVSc Thesis submitted to SDAU, Sardarkrushinagar, Gujarat.

Prajapati Komal, Singh Rana Ranjeet, Chaudhary SS, Kumar Amit and Patel NB (2018a). Effect of extended photoperiod during winter season on production performance and blood biochemistry of Surti goats. Ruminant Science 7(1):109-112.

Prajapati JP, Patel DM, Patel JA, Dhami AJ and Parmar SC (2018b). Relative efficacy of various estrus synchronization protocols for improving fertility in cyclic and acyclic buffaloes. International Journal of Livestock Research 8(7):192-200.

Schallenberger E and Prokopp S (1995). Gonadotrophin and ovarian steroids in cattle IV. Re establishment of the stimulatory feedback action of estradiol-17b on LH and FSH. Acta Endocrinologica 109:44–49.

Selvaraju S, Agarwal SK, Karche SD, Srivastava SK, Majumdar AC and Shanker U (2002). Fertility response and hormonal profiles inn repeat breeding cows treated with insulin. Animal Reproduction Science 73:141-149.

Singh S, Shukla SN and Gupta KK (2019). Fertility response to insulin modified Ovsynch protocol in postpartum acyclic buffaloes. Ruminant Science 8(1):45-48.

Singh Vijay, Misra AK, Kumar Suresh and Kumar Vijay (2015). Effect of growth factors on in vitro maturation and in vitro culture of buffalo oocytes. Ruminant Science 4(2):219-224.

Tanaka T, Nagatani S, Bucholtz DC Ohkura S, Tsukamura H, Maeda K and Foster DL (2000). Central action of insulin regulates pulsatile luteinizing hormone secretion in the diabetic sheep model. Biology of Reproduction 62(5):1256-1261.