12-Title: Efficiency of benzyl penicillin and streptomycin against normal bacterial flora of freshly collected and frozen Kankrej bull semen
Authors: BR Patel and GM Siddiquee
Source: Ruminant Science (2012)-1(1):55-58.
How to cite this manuscript: Patel BR and Siddiquee GM (2012). Efficiency of benzyl penicillin and streptomycin against normal bacterial flora of freshly collected and frozen Kankrej bull semen. Ruminant Science 1(1):55-58.
The objective of the present investigation was to determine the bacterial flora of freshly collected and frozen semen from six clinically healthy Kankrej bulls using ten ejaculates from each bull for ten weeks. In the present study, 60 neat and 60 frozen semen samples were inoculated on brain heart infusion agar (BHI) at 37 °C for 24 hours. Out of 60 neat samples, 13 (21.66 %) samples were positive for gram positive cocci, 22 (36.66 %) samples were positive for gram negative bacilli and remaining 25 (41.66 %) samples were negative for any bacterial growth, whereas out of 60 frozen semen samples, all the samples were negative for bacterial growth. It can be concluded that freshly collected Kankrej bull semen contains normal bacterial flora, which are non pathogenic and sensitive against benzyl penicillin and streptomycin added to the semen dilutors.
Ali S, Abbas D and Payam GD (2011). Frequency of Haemophilus somnus in the semen of bulls in Iran as determined by polymerase chain reaction. Scientific Research and Essays 6(6):1458-1460.
Cruickshank R, Daquid JP, Marmoin BP and Swain RHA (1975). The Practice of Medical Microbiology. 12th Edn, 2-Churchil livingstone, Edinburgh.
Diemer T, Weinder W, Michelmann HW, Sciefer GG, Rovan E and Mayer F (1996). Influence of Escherichia coli on motility parameters of bull spermatozoa in vitro. International Journal of Andrology 19:271-277.
Gandhi A, Sharma M, Dhar P, Katoch V, Thakur A and Kumar R (2008). Isolation of Moraxella bovis from frozen bovine semen and determination of microbial load. Indian Journal of Microbiology 48:405-407.
Griveau JF, Domount E, Renard P, Challegani JP and Lelannou D (1995). Reactive oxygen species lipid peroxidation and enzymatic defence in bull spermatozoa. Journal of Reproduction and Fertility 103:17-26.
Kher HN and Dholakia PM (1984). Bacteriological studies of bovine semen. Indian Journal of Animal Reproduction 5(1):78-84.
Ronald BSM and Prabhakar TG (2001). Bacterial analysis of semen and their antibiogram. Indian Journal Animal Science 71:829-831.
Saxena MS (2000). Veterinary Andrology and Artificial Insemination. CBS Publishers and Distributors, New Delhi pp 89-94.
Shahid HA, Rani W, Muhammad TT, Asghar AK and Muhammad M (2009). Biochemical activities of bacterial species isolated from the frozen semen of cattle. Journal of Agriculture and Social Science 5(4):109-113.
Waltz FA, Foley CW, Herschler RC, Tiffany LW and Liska BJ (2011). Bacteriological studies of Boar semen. Journal of Animal Science 27:1357-1362.