12-Title: Efficiency of benzyl penicillin and streptomycin against normal bacterial flora of freshly collected and frozen Kankrej bull semen

Authors: BR Patel and GM Siddiquee

Source: Ruminant Science (2012)-1(1):55-58.

How to cite this manuscript: Patel BR and Siddiquee GM (2012). Efficiency of benzyl penicillin and streptomycin against normal bacterial flora of freshly collected and frozen Kankrej bull semen. Ruminant Science 1(1):55-58.


The objective of the present investigation was to determine the bacterial flora of freshly collected and frozen semen from six clinically healthy Kankrej bulls using ten ejaculates from each bull for ten weeks. In the present study, 60 neat and 60 frozen semen samples were inoculated on brain heart infusion agar (BHI) at 37 °C for 24 hours. Out of 60 neat samples, 13 (21.66 %) samples were positive for gram positive cocci, 22 (36.66 %) samples were positive for gram negative bacilli and remaining 25 (41.66 %) samples were negative for any bacterial growth, whereas out of 60 frozen semen samples, all the samples were negative for bacterial growth. It can be concluded that freshly collected Kankrej bull semen contains normal bacterial flora, which are non pathogenic and sensitive against benzyl penicillin and streptomycin added to the semen dilutors.


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