13-Title: Biochemical attributes of cattle and buffalo bull semen: A comparative study

Authors: Vijay Pandey, Rajesh Nigam, Atul Saxena, Pawanjit Singh, Ambika Sharma, Dilip Kumar Swain, Lokesh Sharma and Shilpi Dixit

Source: Ruminant Science (2012)-1(1):59-62.

How to cite this manuscript: Pandey Vijay, Nigam Rajesh, Saxena Atul, Singh Pawanjit, Sharma Ambika, Swain Dilip Kumar, Sharma Lokesh and  Dixit Shilpi (2012). Biochemical attributes of cattle and buffalo bull semen: A comparative study. Ruminant Science 1(1):59-62.


The present study was undertaken to establish the baseline semen biochemical indices as well as the difference in two species if any for Hariyana cattle and Bhadawari buffalo bulls. Six fertile, healthy adult Hariyana bulls and Bhadawari buffalo bulls aged between 2 to 4 years were used as semen donors. Twenty four semen samples (four ejaculates of each animals) were collected from bulls by using Artificial Vagina in the morning hours and immediately after collection, seminal plasma was separated by centrifugation. The results showed significantly higher values of AST, ALP, amylase, cholesterol and triglycerides in buffalo bulls as compared to bulls.  


Abdou MSS, El-Guindi MM, El-Menoufy AA and Zaki K (1978). Enzymic profile of the semen of bovines (Bubalus bubalis and Bos taurus). II Parallelism between acid and alakaline phosphatase and various measures of semen quality. Zbl Vet Med A 25:814.

Aguirre SM, Capaul EG, Luca LD and Cinque M (1988). Total and non-prostatic acid phosphatase and alkaline phosphatise activity in bull and ram semen. Verterinaria Argentina 5:282-287.

Andrabi SMH (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reproduction in Domestic Animals 44:552-569.

Brooks DE (1990). Biochemistry of the male accessory glands. In: Marshall’s Physiology of Reproduction. Eds GE Lamming, 4th Edn, Edinburgh, Churchill Livingstone pp 569-690.

Chauhan RAS and Srivastava RK (1973). Enzyme composition of buffalo seminal plasma. Journal of Reproduction and  Fertility 34:165-166.

Ciereszko A, Glogowski J, Strzezek J and Demianowicz W (1992). Low stability of aspartate aminotransferase activity in boar semen. Theriogenology 37:1269-1281.

Cross NL (1998). Role of cholesterol in sperm capacitation. Biology of Reproduction 59:7-11.

Dhami AJ and Kodagali SB (1989). Comparative analysis physicobiochemical attributes in static and motile semen ejaculates from Surti buffalo bulls. Indian Journal of Animal Science 59:544-547.

El-Azab AI, Rakha AM, Aboul-Fadle WS and Ibrahim SS (1983) Interaction of season and nutrition on buffalo semen II-chemical Composition. Egyptian Journal of Veterinary Science 20(2):129-134.

El-Guindi MM and Abdou MSS (1978). Enzymic profile of the semen of bovines (Bubalus bubalis and Bos taurus). III-Amylase (?-1, 4- glucan, 4-glucanohydrolase) activity. Zbl Vet Med A 25: 214.

Gundogan M (2006) Some Reproductive Parameters and. Seminal Plasma Constituents in Relation to Season in Akkaraman and Awassi Rams. Turkish Journal of Veterinary and Animal Science 30: 95-100.

Hafez ESE (1987). Semen evaluation. In: Reproduction in Farm Animals. Eds ESE Hafez, 5th Edn, Philadelphia pp 455-480.

Iqbal M, Samad HA, Yaqub M and Najib-ur-Rehman (1984). Comparative study of cholesterol and total lipids in semen of Nili-Ravi buffalo bulls and sahiwal bulls. Pakistan Veterinary Journal 4:158-160.

Mann T and Lutwak-Mann C (1981). Biochemistry of spermatozoa: chemical and functional correlations in ejaculated semen. In: Male Reproductive Function and Semen. Themes and Trends in Physiological and Biochemical and Investigative Andrology, Eds T Mann and C Lutwak- Mann, Spring -Verlag:  Berlin, pp 195-268.

Pace MM and Graham EF (1970). The release of glutamic oxaloacetic transaminase from bovine spermatozoa as a test method of assessing semen quality and fertility. Biology of Reproduction 3:14-46.

Pesch S, Bergmann M and Bostedt H (2006). Determination of some enzymes and macro and microelements in stallion seminal plasma and their correlations to semen quality. Theriogenology 66(2):307-313.

Pickett BW and Komarek RJ (1966) Lipid and dry weight of bovine seminal plasma and spermatozoa from first and second ejaculates. Journal of Dairy Science 50:742-746.

Tummaruk P, Lundeheim N, Einarsson S and Dalin AM (2000). Reproductive performance of purebred Swedish Landrace and Swedish Yorkshire sows: II, Effect of mating type, weaning-to-first service interval and lactation length. Acta Veterinaria Scandinavica 50:217-224.

Turner RM and McDonnell SM (2003). Alkaline phosphatase in stallion semen: characterization and clinical applications. Theriogenology 60:1-10.

Veerabramhaiah K, Rao AS, Rao VH, Naidu KV and Rao STV (2011). Effect of tris and biociphos-plus extenders on the extracellular enzyme release of phosphatases and transferases in Punganur bull semen. Journal of Advanced Veterinary Research 1:61-64.