3-Title: Diagnosis of rotavirus infection in neonatal calves by ribose nucleic acid polyacrylamide electrophoresis (RNA-PAGE) and double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA)

Authors: DK Kapadiya, DV Joshi, SS Chaudhary, NM Shah, HH Panchasara and BJ Patel

Source: Ruminant Science (2012)-1(1):15-18.

How to cite this manuscript: Kapadiya DK, Joshi DV, Chaudhary SS, Shah NM, Panchasara HH and Patel BJ (2012). Diagnosis of rotavirus infection in neonatal calves by ribose nucleic acid polyacrylamide electrophoresis (RNA-PAGE) and double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA). Ruminant Science 1(1):15-18.


Neonatal calf diarrhoea of rotaviral origin is one of the leading causes of morbidity and mortality posing a serious threat to the dairy industry. In the present study, a total of 70 faecal samples were collected from buffalo calves up to one month of age and screened for rotavirus infection by RNA-PAGE and DAS-ELISA. Of 70 diarrhoeic faecal samples tested by RNA-PAGE, 15 (21.42%) were positive for rotavirus and 17 (24.28%) by DAS-ELISA. All the 15 samples found positive by RNA-PAGE were also found positive by DAS-ELISA, indicating that detection of rotavirus type-A in faecal samples by DAS-ELISA is more sensitive than RNA-PAGE.


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