10-Title: Detection of exou and exos genes among Pseudomonas aeruginosa isolates of animal and human origin
Authors: Rahul Agarwal, Sunil Maherchandani and Dharam Gopal Gupta
Source: Ruminant Science (2013)-2(1):45-49.
How to cite this manuscript: Agarwal Rahul, Maherchandani Sunil and Gupta Dharam Gopal (2013). Detection of exou and exos genes among Pseudomonas aeruginosa isolates of animal and human origin. Ruminant Science 2(1):45-49.
A total of 35 isolates of P. aeruginosa were obtained from 119 clinical samples of animal and human origin were subjected to genotypic charecterization using exoS and exoU gene responsible for ADP-ribosilating enzyme activity and phospholipase activity respectively acting as major genes responsible for virulent activity. Both these genes mostly are mutually exclusive type III effector genes. Out of 35 clinical isolates, 22(62.85%) were found to be positive for exoS gene and 18(51.42%) were found to be positive for exoU gene. Of these 22 isolates having exoS, there were 11 (31.42%) isolates which had both exoS and exoU genes which showed that exoS and exoU genes do not seem to be essentially mutually exclusive. High percentage of isolates were found to have either both the genes or none of them.
Berthelot P, Attree I, Plesiat P, Chabert J, de Bentzmann S, Pozzetto B and Grattard F (2003). Genotypic and phenotypic analysis of type III secretion system in a cohort of Pseudomonas aeruginosa bacteremia isolates: Evidence for a possible association between O serotypes and exo genes. Journal Infectious Disease 188:512-518.
Bodey GP, Bolivar R, Fainstein V and Jadeja L (1983). Infections caused by Pseudomonas aeruginosa. Reviews of Infectious Diseases 5:279-313.
Cowan ST and Steel KJ (1974). In: Cowan and Steel’s manual for the identification of medical bacteria. 2nd Edition Cambridge University Press, Cambridge, New York. pp 90-93.
Feltman H, Schulert G, Khan S, Jain M, Peterson L and Hauser AR (2001). Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosa. Microbiology 147:2659-2669.
Hauser AR (2009). The type III secretion system of Pseudomonas aeruginosa: Infection by injection. Nature 7:654-665.
Hernandez J, Ferrus MA, Hernandez M and Owen RJ (1997). Arbitrary primed PCR fingerprinting and serotyping of clinical Pseudomonas aeruginosa strains. FEMS Immunology Medical Microbiology 17(1):37-47.
Holder I (1993). Pseudomonas aeruginosa burn infections: pathogenesis and treatment. In: Campa M, Bendinelli M and Friedman H (Eds), Pseudomonas aeruginosa as an opportunistic pathogen. Plenum Press, New York, USA. pp 275-295.
Kimata N, Nishino T, Suzuki S and Kogure K (2004). Pseudomonas aeruginosa isolated from marine environments in Tokyo Bay. Microbial Ecology 47:41-47.
Lanotte P, Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A and Quentin R (2004). Genetic features of Pseudomonas aeruginosa isolates from cystic ûbrosis patients compared with those of isolates from other origins. Journal Medical Microbiology 53:73-81
Masaadeh HA and Jaran AS (2009). Incident of Pseudomonas aeruginosa in post-operative wound infection. American Journal Infectious Disease 5(1):1-6.
Pollack M (2000). Pseudomonas aeruginosa. In: Mandell GL, Bennett JE and Dolin R (Eds.) Principles and practice of infectious diseases. Churchill Livingstone, New York. pp 2310-2335.
Rahme LG, Ausubel FM, Cao H, Drenkard E, Goumnerov BC, Lau G W, Mahajan-Miklos S, Plotnikova J, Tan MW, Tsongalis J, Walendziewicz CL and Tompkins RG (2000). Plants and animals share functionally common bacterial virulence factors. Proceedings of National Academy of Sciences of the United States of America 97(16):8815-8821.
Rodriguez CN, Rodriguez-Morales A.J, Garcia A, Pastran B and Meijomil P (2006). Antimicrobial resistance of Pseudomonas aeruginosa strains isolated from surgical infections in a 7-year period at a general hospital in venezuela. Surgical Infections 7(3): 269-273.
Sadikot RT, Blackwell TS, Christman JW and Prince AS (2005).Pathogen-host interactions in Pseudomonas aeruginosa pneumonia. American Journal of Respiratory and Critical Care Medicine 171:1209-1223.
Shaver CM and Hauser AR (2004). Relative Contributions of Pseudomonas aeruginosa ExoU, ExoS, and ExoT to Virulence in the Lung. Infectious Immunology 72(12):6969–6977.
Spilker T, Coenye T, Vandamme P and LiPuma JJ (2004). PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. Journal Clinical Microbiology 42(5):2074-2079.
Stewart R, Wiehlmann L, Ashelford KE, Preston SJ and Frimmersdorf E (2011). Genetic characterization indicates that a specific subpopulation of Pseudomonas aeruginosa is associated with keratitis infections. Journal clinical microbiology 49(3):993-1003.
Vallis AJ, Finck-Barbancon V, Yahr TL and Frank DW (1999). Biological effects of Pseudomonas aeruginosa type III-secreted proteins on CHO cells. Infectious Immunology 67:2040-2044.
Van Delden CV (2004). Virulence factors in Pseudomonas aeruginosa. In: JL Ramos (Eds), Pseudomonas: Virulence and Gene Regulation, Vol 2. Kluwer Academic/Plenum Publishers, New York. pp 3-45.
Winstanley C, Kaye SB, Neal TJ, Chilton HJ, Miksch S and Hart CA (2005). Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis. Journal Medical Microbiology 54:519-526.