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13 - Anandpub

13-Title: Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes

Authors: EM Sadeesh, Fozia Shah, AK Balhara, SMK Thirumaran, Sunesh Balhara and   PS Yadav

Source: Ruminant Science (2013)-2(1):59-62.

How to cite this manuscript: Sadeesh EM, Shah Fozia, Balhara AK, Thirumaran SMK, Balhara Sunesh and   Yadav PS (2013).  Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes. Ruminant Science 2(1):59-62.

Abstract

The aim of this study was to determine the effect of thiol compound, beta mercaptoethanol (β- ME) on the in vitro maturation (IVM) of oocytes. Good quality of cumulus oocyte complexes   were matured  in two groups of maturation media viz. TCM 199 + 10% FBS + PMSG (20 IU/ml) + hCG(10 IU/ml) + Sodium pyruvate (0.80 mM) + L-glutamine (2 mM) + Gentamicin sulphate (50 µg/ml) without b-ME (Group 1) and  supplemented with 100 µM b-ME (Group 2)  for 24 h in CO2 incubator. The present result revealed that a significant improvement in cumulus expansion and nuclear maturation was observed when buffalo oocytes were matured in maturation medium with b-ME. In conclusion, supplimentation of   b-ME in culture medium had a beneficial effect on in vitro maturation of buffalo oocytes. 

References

Beheshti R, Mohammadi-Roshandeh A, Giasi-Ghalehkandi J, Ghaemmaghami S and Houshangi AF (2011). Effect of antioxidant supplements on in vitro maturation of bovine oocyte. Advances in Environmental Biology 5:1473-1475.

Chauhan MS, Singla SK, Palta P, Manik RS and Tomar OS (1998). Development of in vitro produced buffalo (Bubalus bubalis) embryos in relation to time. Asian Australasian Journal of Animal Sciences 11:398-403.

De Matos DG, Gasparrini B, Pasqualini SR and Thompson JG (2002). Effect of glutathione synthesis stimulation during in vitro maturation of ovine oocytes on embryo development and intracellular peroxide content. Theriogenology  57:1443-1451.

Desousa PA, Westhusin ME and Watson AJ (1998). Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos. Molecular Reproduction and Development 49:119-130.

Fagbohun CF and Downs SM (1990). Maturation of the mouse oocyte cumulus cell complex: stimulation by Lectins. Biology of Reproduction 42:413-423.

Gasparrini B, Sayoud H, Neglia G, De-Matos DG, Donnay I and Zicarelli L (2003). Glutathione synthesis during in vitro maturation of buffalo (Bubalus bubalis) oocytes: Effects of cysteamine on embryo development. Theriogenology 60:943-952.

Gordon I (2003). Laboratory Production of Cattle Embryos: Maturing the Oocyte. 2nd Edn, Cambridge, MA 02138, CABI Publishing

Kobayashi M, Lee ES and Fukui Y (2006). Cysteamine or β-mercaptoethanol added to a defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology 65:1191-1199.

Lafleur MVM, Hoorweg JJ, Joenje H, Westmijze EJ and Retel J (1994). The ambivalent role of glutathione in the protection of DNA against singlet oxygen. Free Radical Research 21:9-17

Racedo S, Herrmann D, Wrenzycki C, Salamone D and Niemann H (2007). Effects of follicle size and stage of maturation on mRNA expression in bovine in vitro matured oocytes. Reproduction Fertility and Development 19:291.

Sawai K, H Funahashi and K Niwa (1997). Stage specific requirement of Cysteine during in vitro maturation of porcine oocytes for glutathione synthesis associated with male pronuclear formation. Biology of Reproduction 57:1-6.

Shah RA, George A, Singh MK, Kumar D, Chauhan MS, Manik R, Palta P, Singla SK (2008). Handmade cloned buffalo (Bubalus bubalis) embryos: Comparison of different media and culture systems. Cloning Stem Cells 10:435–442.

Yoshida M, K Ishigaki, T Nagai, M Chikyu and VG Pursel (1993). Glutathione concentration during maturation and fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus.Biology of Reproduction 49:89-94.