13-Title: Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes

Authors: EM Sadeesh, Fozia Shah, AK Balhara, SMK Thirumaran, Sunesh Balhara and   PS Yadav

Source: Ruminant Science (2013)-2(1):59-62.

How to cite this manuscript: Sadeesh EM, Shah Fozia, Balhara AK, Thirumaran SMK, Balhara Sunesh and   Yadav PS (2013).  Effect of thiol compound supplemented culture medium on in vitro maturation of buffalo oocytes. Ruminant Science 2(1):59-62.


The aim of this study was to determine the effect of thiol compound, beta mercaptoethanol (β- ME) on the in vitro maturation (IVM) of oocytes. Good quality of cumulus oocyte complexes   were matured  in two groups of maturation media viz. TCM 199 + 10% FBS + PMSG (20 IU/ml) + hCG(10 IU/ml) + Sodium pyruvate (0.80 mM) + L-glutamine (2 mM) + Gentamicin sulphate (50 µg/ml) without b-ME (Group 1) and  supplemented with 100 µM b-ME (Group 2)  for 24 h in CO2 incubator. The present result revealed that a significant improvement in cumulus expansion and nuclear maturation was observed when buffalo oocytes were matured in maturation medium with b-ME. In conclusion, supplimentation of   b-ME in culture medium had a beneficial effect on in vitro maturation of buffalo oocytes. 


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