14-Title: Effect of semen additives on leakage of certain enzymes during cryopreservation of Kankrej semen
Authors: BR Patel and GM Siddiquee
Source: Ruminant Science (2013)-2(1):63-66.
How to cite this manuscript: Patel BR and Siddiquee GM (2013). Effect of semen additives on leakage of certain enzymes during cryopreservation of Kankrej semen. Ruminant Science 2(1):63-66.
The objective of the present investigation was to study the effect of semen extender additives on extracellular release of enzymes subsequent to semen dilution, equilibration and thawing of cryopreserved Kankrej bull semen. Total 60 ejaculates, 10 each from 6 mature Kankrej bulls were collected and divided in 3 equal aliquots after diluting semen with TFYG diluent. Then first aliquot was added with EDTA (0.1%), second with caffeine (0.5%) as semen diluent additives and third aliquot was processed without additive and served as control. All the 3 aliquots were processed for freezing of semen at ultra low temperature to see the release of aspartate amino transferase (AST), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) at post diluted, post equilibration and post thaw stages of semen preservation. The release of AST, LDH and ALP enzymes increased in ascending order from post diluted to post thaw stages of semen preservation in control, EDTA and caffeine groups but significantly (P<0.05) lesser leakage of all enzymes were observed in EDTA and caffeine groups as compared to control group at post equilibration and post thaw stages of semen preservation. On comparison the EDTA was superior at post diluted and post equilibration stages whereas caffeine was found superior at post thaw stage. It can be concluded that addition of EDTA and caffeine as semen extender additives prevented leakage of AST, LDH and ALP enzymes during semen preservation as compared to diluent without any additive.
Annual Progress Report (2009). Livestock Research Station, SDAU, Sardarkrushinagar, Banaskatha (District), Gujarat.
Belorkar PM, Dhami AJ and Kodagali SB (1988). Physico-biochemical semen characteristics of good and poor freezability crossbred bulls. Indian Journal Animal Science 58(12):1419-1423.
Dhami AJ and Kodagali SB (1988). Seminal characteristics and their interrelation-ships in Surti buffalo. Indian Veterinary Journal 65(1):61-64.
Dhami AJ and Sahni KL (1993). Effect of extenders, additives and deep freezing on the leakage of lactic dehydrogenase from cattle and buffalo spermatozoa. Indian Journal Animal Science 63(3):251-256.
Dhami AJ, Mohan G and Sahni KL (1995). Seminal LDH and trace elements profile in the static versus motile ejaculates of Friesian and Murrah buffalo bulls. International Journal of Animal Science 10(1):151-156.
Dhami AJ, Shelke VB, Virani AC, Kavani FS and Dave RR (2002). Transaminases and phosphatase activity of blood and seminal plasma in breeding bulls of different breeds. Indian Journal of Animal Reproduction 23(1):35-37.
Kakar ML and Arora KL (1973). Studied on semen phosphatase and transaminases of young HF x Hariana and BS x Hariana crossbred bulls as affected by post-pubertal age. Indian Journal Animal Science 43(2):91-95.
Mohan G, Sahni KL, Dhami AJ and Tripathi RP (1992). Prediction of freezability on the basis of seminal plasma enzymatic profiles in Murrah, Friesian and crossbred bulls. Indian Journal Animal Science 62(9):811-815.
Mohanty DN and Anasari MR (2004). Studies on certain seminal plasma enzymes of crossbred bulls in relation to freezability of semen. Indian Journal Animal Science 25:29-32.
Pangawkar GR, Sharma RD and Sharma R (1988). Phosphatase and transaminase activity in the seminal plasma of bulls in relation to freezability of semen. Theriogenology 26(6):1393-1399.
Reitman S and Franken S (1957). A colorimetric method for the determination of serum glutamic
oxaloacetic transaminase and serum glutamic pyruvic transaminase. American Journal of Clinical Pathology 28:56-60.
Shelke VB and Dhami AJ (2001). Comparative evaluation of physicomorphological of buffalo semen extende in CAW extender. Proceedings of IInd World Buffalo Congress, New Delhi, India, 2:89-93.
Singh G, Bujarbaruah KM and Mondal SK (2010). The effect of storage temperature on enzyme activity in Mithun. Indian Veterinary Journal 87:924-925.
Smith JT, Mayer DT and Merilin GP (1957). Seasonal variations of succinic dehydrogenase activity of bovine spermatozoa. Journal of Dairy Science 40(4):516-518.
Snedecor GW and Cochran WG (1994). Statistical Methods. 8th Edn, Affiliated East-West Press, New Delhi, India.
Sori H, Prasad S and Zewdie E (2006). Seminal characterization of some elite Ethiopian indigenous breeds of bull. Indian Journal of Animal Reproduction 27(2):54-60.
Stalloup T and Hayden JS (1960). Lactic dehydrogenase activity in bovine semen. Journal of Dairy Science 43(2):266-269.
Veerabramhaiah KA, Seshagiri RA, Rao VH, Venugopal K, Naidu ST and Rao V (2011). Effect of tris and biociphos-plus extenders on the extracellular enzyme release of phosphatases and transferases in Punganur bull semen. Journal of Advance Veterinary Research 1:61-64.