2-Title: Generation of parthenogenetic embryos by ethanol activation in buffalo
Authors: Gopal Puri and Sadhan Bag
Source: Ruminant Science (2014)-3(1):5-7.
How to cite this manuscript: Puri Gopal and Bag Sadhan (2014). Generation of parthenogenetic embryos by ethanol activation in buffalo. Ruminant Science 3(1):5-7.
The present experiment was carried out to generate parthenogenetic embryo by ethanol activation of oocytes in buffalo. Oocytes were isolated from ovaries brought from local slaughter house. Isolated good quality of oocytes were matured in maturation media and kept in CO2 incubator for 24 hrs. The matured oocyte were activated by exposure to 7% ethanol for 5 minutes followed by incubation in 2mM 6-DMAP for 4 hr. After incubation in 6-DMAP, activated oocytes were finally shifted to mSOF media and kept in CO2 incubator for 24 hrs. The cleavage rate of oocytes was observed after 48-72 hr and 7 days. A total no. of 124 oocytes was used for ethanol activation and subsequent cleavage rate of activated oocytes were recorded. Result showed that overall cleaving percentage of oocytes was 49.80% and the development of embryos of 2 cell, 4 cell, 8 cell, morulae and blastocyst stages were 8.06%, 17.74%, 16.12%, 7.25% and 1.61%, respectively. It can be concluded that parthenogenetic embryos leads to haploid embryo production but fail to develop to term and exhibit phenotypic abnormalities might be due to inherently limited in their developmental capacity.
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