characterization of extracellular domain of glycoprotein e gene of an Indian isolate of bovine herpes virus-1

4-Title: characterization of extracellular domain of glycoprotein e gene of an Indian isolate of bovine herpes virus-1

Authors: Ananthakrishna LR, Aman Kamboj, Mohini Saini and Praveen K Gupta

Source: Ruminant Science (2015)-4(1):15-20.

How to cite this manuscript: Ananthakrishna LR, Kamboj Aman, Saini Mohini and Gupta PK (2015). Characterization of extracellular domain of glycoprotein e gene of an Indian isolate of bovine herpes virus-1. Ruminant Science 4(1):15-20.



Bovine herpesvirus (BoHV-1, a member of the subfamily Alphaherpesvirinae of the family Herpesviridae is an economically important pathogen of cattle which is associated with infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) and other manifestations. Among the several mirror glycosylated protein of BoHV-1, the glycoprotein E (gE) is phosphorylated and is associated with immune response and virulence. In this study, the extracellular domain of gE from an Indian isolate of BoHV-1 was PCR amplified, cloned and sequence was analyzed in silico for various characteristics such as repertoire of nucleotide information, secondary structure prediction and identification of potential glycosylation and phosphorylation sites. Multiple sequence alignment with that of other BoHV-1 strains revealed eight changes at nucleotide level which resulted in two non-synonymous substitutions 273(R-H) and  341 (V-A) at amino acid level. Since these changes are within the same class of amino acids, the predicted structure of the extracellular domain of gE was found similar to BoHV-1 Cooper strain and conserved among other BoHV-1 strains compared.


Ackermann M and Engels M (2006). Pro and contra IBR-eradication. Veterinary Microbiology 113: 293-302.

Brum, MCS, Coats C, Sangena RB, Doster A, Jones C and Chowdhury SI (2009). Bovine herpesvirus type 1 (BoHV-1) anterograde neuronal transport from trigeminal ganglia to nose and eye requires glycoprotein E. Journal of Neurovirology 15:196-201.

Chung T, Niemela SL and Miller RH (1989). One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences of the United States of America 86:2172-2175.

Gibbs EPJ and Rweyemamu MM (1977). Bovine herpesviruses. Part I. Bovine herpesvirus-1, Veterinary Bulletin 47:317-343.

Graham DA (2013). Bovine herpes virus-1 (BoHV-1) in cattle – A review with emphasis on reproductive impacts and the emergence of infection in Ireland and the United Kingdom. Irish Veterinary Journal 66:15.

Gupta PK and Rai A (1993). Restriction enzyme analysis of bovineherpesvirus-1 DNA from an Indian isolate. Biochemistry and Molecular Biology International 29:889-898.

Han J, Chadha P, Starkey JL and Wills JW (2012). Function of glycoprotein E of herpes simplex virus requires coordinated assembly of three tegument proteins on its cytoplasmic tail. Proceedings of the National Academy of Sciences of the United States of America 109:19798-19803.

Kelley LA and Sternberg MJE (2009). Protein structure prediction on the web: A case study using the Phyre server. Nature protocols 4:363-371.

Kratchmarov R, Kramer T, Greco TM, Taylor MP, Ch’ng TH, Cristea IM and Enquist LW (2013). Glycoproteins gE and gI are required for efficient KIF1A-Depend anterograde axonal transport of alpha herpes virus particles in neurons. Journal of Virology 87:9431-9440.

Mehrotra ML, Rajya BS and Kumar S (1976). IBR keratoconjunctivitis in calves. Indian Journal of Veterinary Pathology 1:70-73.

Miller JM, Van Der Maaten MJ and Whetstone CA (1988). Effects of a bovine herpes virus-1 isolate on reproductive function in heifers: Classification as a type-2 (infectious pustular vulvovaginitis) virus by restriction endonuclease analysis of viral DNA. American Journal of Veterinary Research 49:1653-1656.

Muylkens B, Thiry J, Kirten P, Schynts F and Thiry E (2007). Bovine herpes virus 1 infection and infectious bovine rhinotracheitis. Veterinary Research 38:181-209.

Nandi S, Kumar M, Manohar M and Chauhan RS (2009). Bovine herpes virus infections in cattle. Animal Health Research Reviews 10:85-98.

Romera S, Puntel M, Quattrocchi V, Zajac P, Zamorano P, Viera J, Carrillo C, Chowdhury S, Borca M and Sadir A (2014). Protection induced by a glycoprotein E-deleted bovine herpes virus type 1 marker strain used either as an inactivated or live attenuated vaccine in cattle. BMC Veterinary Research 10:8.

Shaw A, Braun L, Frew F, Hurley D, Rowland R and Chase C (2000). A Role for Bovine Herpesvirus 1 (BHV-1) Glycoprotein E (gE) tyrosine phosphorylation in replication of BHV-1 wild type virus but not BHV-1 gE deletion mutant virus. Virology 268(1):159-66.

Tikoo SK, Campos M and Babiuk LA (1995). Bovine Herpesvirus 1(BHV-1: Biology, pathogenesis and control. Advances in Virus Research 45:191-223.